{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:02:40Z","timestamp":1767960160998,"version":"3.49.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,10,7]],"date-time":"2002-10-07T00:00:00Z","timestamp":1033948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rearrangements involving the <jats:italic>MLL<\/jats:italic> gene at chromosome band 11q23 are common in infant acute myeloid leukemias (AMLs). We recently encountered an infant patient with rapidly progressive AML whose leukemic cells harbored a previously undescribed <jats:italic>MLL<\/jats:italic> rearrangement involving an inversion of 11q [inv(11)(q14q23)]. We used panhandle PCR to determine that this rearrangement juxtaposed the <jats:italic>MLL<\/jats:italic> (<jats:italic>M<\/jats:italic>ixed\u2010<jats:italic>L<\/jats:italic>ineage <jats:italic>L<\/jats:italic>eukemia) gene to the <jats:italic>CALM<\/jats:italic> (<jats:italic>C<\/jats:italic>lathrin <jats:italic>A<\/jats:italic>ssembly <jats:italic>L<\/jats:italic>ymphoid <jats:italic>M<\/jats:italic>yeloid leukemia) gene at 11q14\u2013q21. The CALM protein participates in recruitment of clathrin to internal membrane surfaces, thereby regulating vesicle formation in both endocytosis and intracellular protein transport. Intriguingly, <jats:italic>CALM<\/jats:italic> has been identified in other cases of AML as a translocation partner for the <jats:italic>AF10<\/jats:italic> gene, which has independently been found to be an <jats:italic>MLL<\/jats:italic> partner in AML. We identified the <jats:italic>MLL<\/jats:italic>\u2010<jats:italic>CALM<\/jats:italic> fusion transcript (but not the reciprocal <jats:italic>CALM<\/jats:italic>\u2010<jats:italic>MLL<\/jats:italic> transcript) in leukemia cell RNA by RT\u2010PCR. The predicted 1803 amino acid MLL\u2010CALM fusion protein includes amino\u2010terminal MLL domains involved in transcriptional repression, and carboxy\u2010terminal CALM\u2010derived clathrin\u2010binding domains. The genomic breakpoint in <jats:italic>MLL<\/jats:italic> is in the 7th intron (within the breakpoint cluster region); the corresponding <jats:italic>CALM<\/jats:italic> breakpoint is in the 7th <jats:italic>CALM<\/jats:italic> intron. In contrast, breakpoints in <jats:italic>CALM<\/jats:italic>\u2010<jats:italic>AF10<\/jats:italic> translocations lie in the 17th\u201319th <jats:italic>CALM<\/jats:italic> introns (30 kb downstream); also, in these translocations, <jats:italic>CALM<\/jats:italic> provides the 5\u2032 end of the fusion transcript. Together with its previously recognized association with <jats:italic>AF10<\/jats:italic> in AML, the identification of <jats:italic>CALM<\/jats:italic> as an <jats:italic>MLL<\/jats:italic> fusion partner suggests that interference with clathrin\u2010mediated trafficking pathways may be an underappreciated mechanism in leukemogenesis. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10136","type":"journal-article","created":{"date-parts":[[2002,12,3]],"date-time":"2002-12-03T13:50:22Z","timestamp":1038923422000},"page":"26-36","source":"Crossref","is-referenced-by-count":35,"title":["A novel chromosomal inversion at 11q23 in infant acute myeloid leukemia fuses <i>MLL<\/i> to <i>CALM,<\/i> a gene that encodes a clathrin assembly protein"],"prefix":"10.1002","volume":"36","author":[{"given":"Daniel S.","family":"Wechsler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars 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