{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T05:24:55Z","timestamp":1697520295629},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,5,7]],"date-time":"2003-05-07T00:00:00Z","timestamp":1052265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Medical Research Fund of Tampere University Hospital, Tampere, Finland"},{"name":"Swedish Cancer Society; Lion's Cancer Research Foundation, Uppsala University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>B\u2010cell chronic lymphocytic leukemia (CLL) is not a uniform disease entity; approximately half of the CLL cases have undergone immunoglobulin V<jats:sub>H<\/jats:sub> gene hypermutation, whereas the other half display unmutated V<jats:sub>H<\/jats:sub> genes. We investigated genome changes in 12 hypermutated cases (M\u2010CLL) and 22 unmutated cases (UM\u2010CLL) by use of comparative genomic hybridization, G\u2010banding, and multicolor fluorescence in situ hybridization (m\u2010FISH) after optimal mitogen stimulation and FISH analysis of typical CLL aberrations: 11q deletion, 13q deletion, and trisomy 12. Very high frequencies of aberrations were found in both groups: 82% in UM\u2010CLL and 83% in M\u2010CLL. Deletions of 11q and 13q were equally distributed in M\u2010CLL and UM\u2010CLL. However, larger aberrations detectable by CGH, trisomy 12, and complex aberrations were less frequent in M\u2010CLL than in UM\u2010CLL. These observations led to a hypothesis that unmutated and mutated CLL have different biological Backgrounds, given that large and\/or complex chromosomal aberrations and hypermutation of the CLL progenitor cells tend to be mutually exclusive. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10227","type":"journal-article","created":{"date-parts":[[2003,6,6]],"date-time":"2003-06-06T10:07:35Z","timestamp":1054894055000},"page":"417-420","source":"Crossref","is-referenced-by-count":13,"title":["More extensive genetic alterations in unmutated than in hypermutated cases of chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"37","author":[{"given":"Ritva","family":"Karhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard","family":"Tobin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf","family":"Thunberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leena","family":"Vilpo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christer","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakari","family":"Knuutila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Rosenquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juhani","family":"Vilpo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,5,7]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1840"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402528"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1848"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(90)90079-P"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870100403"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199708)19:4<286::AID-GCC12>3.0.CO;2-E"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10074"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00050-4"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015103"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1999.tb01880.x"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.4.1177.h81602001177_1177_1184"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90009-4"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402537"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.6.2262"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402583"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0609.2003.02838.x"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401405"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.10227","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.10227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:29:39Z","timestamp":1697437779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.10227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,7]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1002\/gcc.10227"],"URL":"https:\/\/doi.org\/10.1002\/gcc.10227","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,7]]}}}