{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:38:09Z","timestamp":1789749489713,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,6,27]],"date-time":"2003-06-27T00:00:00Z","timestamp":1056672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The high mobility group gene, <jats:italic>HMGA2<\/jats:italic>, is frequently expressed in uterine leiomyomata (UL) with chromosomal rearrangements of 12q15. In contrast, <jats:italic>HMGA2<\/jats:italic> expression has not been detected in karyotypically normal UL or in myometrium, but has been detected in these tissues after culture. To characterize further the expression pattern of <jats:italic>HMGA2<\/jats:italic>, we assessed <jats:italic>HMGA2<\/jats:italic> expression by RT\u2010PCR followed by Southern blot hybridization, and by real\u2010time PCR in three tissue panels: (1) primary myometrial cultures, (2) uncultured tissue from 15 karyotypically normal samples consisting of eleven 46,XX UL and four matched myometrial specimens, and (3) uncultured tissue from ten UL with 12q15 rearrangements and three matched myometrial specimens. <jats:italic>HMGA2<\/jats:italic> expression was detected in all samples from the three panels. The level of <jats:italic>HMGA2<\/jats:italic> expression in karyotypically normal UL was similar to the level of expression in myometrium; however, it was significantly less than the level measured in UL with 12q15 rearrangements. This expression analysis by use of detection methods of different sensitivities underscores the importance of studies of <jats:italic>HMGA2<\/jats:italic> expression in uncultured tissues and of careful interpretation of results from experiments on cultured cells. Moreover, detection of <jats:italic>HMGA2<\/jats:italic> expression in myometrium and in UL without 12q15 rearrangements, tissues previously thought not to express <jats:italic>HMGA2<\/jats:italic>, suggests that <jats:italic>HMGA2<\/jats:italic> expression is required in normal adult myometrial physiology. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10240","type":"journal-article","created":{"date-parts":[[2003,7,17]],"date-time":"2003-07-17T10:09:30Z","timestamp":1058436570000},"page":"68-79","source":"Crossref","is-referenced-by-count":71,"title":["<i>HMGA2<\/i> expression in uterine leiomyomata and myometrium: Quantitative analysis and tissue culture studies"],"prefix":"10.1002","volume":"38","author":[{"given":"Karen L.","family":"Gross","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David 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