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Fourteen differentially expressed genes in nasopharyngeal epithelial tissue were obtained. Among these genes, <jats:italic>LPLUNC1<\/jats:italic> and <jats:italic>SPLUNC1<\/jats:italic> were confirmed to be specifically expressed in nasopharyngeal epithelial tissue and the trachea. A novel transcript of <jats:italic>SPLUNC1<\/jats:italic>, which we designate <jats:italic>NASG<\/jats:italic>, was found. We also combined SSH and cDNA microarray hybridization to identify genes whose expressions were altered in nasopharyngeal carcinoma (NPC). We used NPC cell line HNE1 and primary human embryo nasopharyngeal epithelial cells in one SSH experiment, and NPC biopsies and normal adult nasopharyngeal epithelial tissue in another. Some 1,200 SSH inserts from four subtractive cDNA libraries were arrayed onto nylon membranes by use of robotic printing. Differential gene expression was verified by hybridizing of the membranes with radioactively labeled first\u2010strand cDNA from NPC cell line HNE1, primary human embryo nasopharyngeal epithelial cells, NPC biopsies, and normal adult nasopharyngeal epithelial tissue. Seventeen differentially expressed genes in NPC were obtained. Among these genes, we identified <jats:italic>SPLUNC1<\/jats:italic> and <jats:italic>LPLUNC1<\/jats:italic> to be down\u2010expressed in NPC biopsies (34\/48, 33\/48). \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10247","type":"journal-article","created":{"date-parts":[[2003,7,17]],"date-time":"2003-07-17T10:09:30Z","timestamp":1058436570000},"page":"80-90","source":"Crossref","is-referenced-by-count":58,"title":["Identification of tissue\u2010specific genes in nasopharyngeal epithelial tissue and differentially expressed genes in nasopharyngeal carcinoma by suppression subtractive hybridization and cDNA 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