{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:50:13Z","timestamp":1788871813897,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,9,11]],"date-time":"2003-09-11T00:00:00Z","timestamp":1063238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dr. Mildred Scheel Stiftung, Apoptoseschwerpunkt"},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB 518"],"award-info":[{"award-number":["SFB 518"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trp53<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    mice overexpressing\n                    <jats:italic>Tgf\u03b1<\/jats:italic>\n                    in a pancreas\u2010specific manner represent a well\u2010established animal model for pancreatic cancer. In this study we analyzed 38 pancreatic adenocarcinomas of these mice for secondary genomic changes by comparative genomic hybridization (CGH), loss of heterozygosity (LOH) analysis, real\u2010time PCR, and methylation\u2010specific analysis. CGH screening of the tumors revealed a recurrent pattern of genomic changes. In more than 50% of the tumors, chromosome 11 was affected. The gain of the proximal part spans about 16 cM, including the genes for\n                    <jats:italic>Egfr<\/jats:italic>\n                    ,\n                    <jats:italic>Rel<\/jats:italic>\n                    , and\n                    <jats:italic>Stk10<\/jats:italic>\n                    . The distal part of chromosome 11, which contains the\n                    <jats:italic>Trp53<\/jats:italic>\n                    locus, was deleted. LOH analysis proved that almost all tumors segregate the wild\u2010type\n                    <jats:italic>Trp53<\/jats:italic>\n                    allele. The\n                    <jats:italic>Cdkn2a<\/jats:italic>\n                    locus on chromosome 4 was inactivated by hypermethylation in 55% of all tumors. In addition, two other changes were detected in a mutually exclusive manner: overrepresentation of part of chromosome 15, or more rarely, loss of the distal part of chromosome 14. Together these data suggest the induction of a uniform pattern of secondary genomic changes in this transgenic tumor model for pancreatic cancer. \u00a9 2003 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/gcc.10285","type":"journal-article","created":{"date-parts":[[2003,9,19]],"date-time":"2003-09-19T06:39:10Z","timestamp":1063953550000},"page":"240-248","source":"Crossref","is-referenced-by-count":16,"title":["Pattern of secondary genomic changes in pancreatic tumors of\n                    <i>Tgf<\/i>\n                    \u03b1\/\n                    <i>Trp53<\/i>\n                    <sup>+\/\u2212<\/sup>\n                    transgenic mice"],"prefix":"10.1002","volume":"38","author":[{"given":"Bettina","family":"Schreiner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee 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