{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:40:34Z","timestamp":1791096034365,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,8,3]],"date-time":"2001-08-03T00:00:00Z","timestamp":996796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o Calouste Gulbenkian."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitochondrial DNA (mtDNA) large deletions and mtDNA mutations have been demonstrated in various types of human cancer. The relationship between the occurrence of such alterations and the nuclear microsatellite instability (MSI) status of the neoplastic cells remains controversial. In an attempt to clarify the situation in gastric carcinoma, we studied, by PCR\/SSCP and sequencing, five mitochondrial genes and two D\u2010loop regions in 32 gastric carcinomas that had been previously screened for MSI and mitochondrial common deletion. MtDNA alterations were detected in 26 carcinomas (81%). All the mtDNA mutations, which occurred mainly in the D\u2010loop and ND1 and ND5 genes, were transitions. D\u2010loop alterations (insertions and\/or deletions) were not significantly associated with mutations in the coding regions. There was a trend towards an inverse relationship between the occurrence of mitochondrial common deletion and mtDNA mutations. No significant relationship was observed between MSI status and mtDNA mutations, whereas the mitochondrial common deletion appeared to be almost exclusively restricted to MSI\u2010negative tumors. The latter finding\u2014almost no gastric carcinoma with MSI\u2010positive phenotype has large deletions of mtDNA\u2014needs to be confirmed in a larger series and in tumors from other organs. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.1175","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T19:56:46Z","timestamp":1030305406000},"page":"136-143","source":"Crossref","is-referenced-by-count":94,"title":["Microsatellite instability, mitochondrial DNA large deletions, and mitochondrial DNA mutations in gastric 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