{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T18:27:53Z","timestamp":1769970473546,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T00:00:00Z","timestamp":1077753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A chromosomal translocation involving the <jats:italic>MYC<\/jats:italic> gene is characteristic of Burkitt lymphoma (BL) and represents a molecular disease marker with diagnostic and clinical implications. The detection of <jats:italic>MYC<\/jats:italic> breakpoints is hampered by technical problems, including the distribution of the breakpoints over a very large genomic region of approximately 1,000 kb. In this article, we report on the testing and validation of a segregation fluorescence in situ hybridization (FISH) assay for <jats:italic>MYC<\/jats:italic> breakpoints on a large series of BLs. A contig of overlapping genomic clones was generated, and two probe sets flanking the <jats:italic>MYC<\/jats:italic> gene were selected. Both probe sets were tested in an interphase FISH segregation assay on 8 B\u2010cell lymphoma cell lines and 32 lymphoma samples with proved 8q24\/<jats:italic>MYC<\/jats:italic> abnormalities and validated in 47 BLs from The Netherlands, Brazil, and Uganda. <jats:italic>MYC<\/jats:italic> translocation breakpoints were identified in 98% of the tumors of the test series and in 89% of the cases of the validation series. In 89% of all positive samples, the breakpoints were located between 190 kb 5\u2032 and 50 kb 3\u2032 of <jats:italic>MYC<\/jats:italic>. Nine cases had more distant breakpoints, and in one patient an insertion of <jats:italic>MYC<\/jats:italic> into the IGH region was detected. In two of the three BLs lacking CD10 expression, no breakpoint could be detected, suggesting that CD10 is a discriminative marker of BL. We did not find consistent differences between BL and atypical BL in incidence of an <jats:italic>MYC<\/jats:italic> breakpoint. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20009","type":"journal-article","created":{"date-parts":[[2004,3,17]],"date-time":"2004-03-17T00:02:13Z","timestamp":1079481733000},"page":"10-18","source":"Crossref","is-referenced-by-count":51,"title":["Interphase fluorescence in situ hybridization for detection of 8q24\/MYC breakpoints on routine histologic sections: Validation in Burkitt lymphomas from three geographic regions"],"prefix":"10.1002","volume":"40","author":[{"given":"Eugenia","family":"Haralambieva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ed","family":"Schuuring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Rosati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carel","family":"van Noesel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patty","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge","family":"Appel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen","family":"Guikema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Wabinga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Fernando Bleggi\u2010Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"King","family":"Lam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"van den Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens","family":"Mellink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shama","family":"van Zelderen\u2010Bhola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Kluin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1182\/blood.V88.3.985.bloodjournal883985","article-title":"Application of long\u2010distance polymerase chain reaction to detection of junctional sequences created by chromosomal translocation in mature B\u2010cell neoplasms","volume":"88","author":"Akasaka T","year":"1996","journal-title":"Blood"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65463-6"},{"key":"e_1_2_6_4_1","first-page":"601","article-title":"Histopathological definition of Burkitt's tumour","volume":"40","author":"Berard C","year":"1969","journal-title":"Bull WHO"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.18004619704"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02241.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.24.7824"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65727-6"},{"key":"e_1_2_6_9_1","first-page":"181","volume-title":"World Health Organization Classification of Tumours. 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