{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:35:15Z","timestamp":1790973315299,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T00:00:00Z","timestamp":1080259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The translocation t(12;15)(p13;q25), in which the <jats:italic>ETV6<\/jats:italic> gene from chromosome 12 is rearranged with the <jats:italic>NTRK3<\/jats:italic> gene from chromosome 15, has recently been identified in secretory breast carcinoma (SBC). This fusion gene was initially described in congenital fibrosarcoma and congenital mesoblastic nephroma. The biological consequence of this translocation is the expression of a chimeric protein tyrosine kinase with potent transforming activity. To assess the frequency of t(12;15)(p13;q25) in breast cancer, we developed complementary probe sets (fusion and split\u2010apart probes) for the detection of this translocation by fluorescence in situ hybridization (FISH) in paraffin\u2010embedded, formalin\u2010fixed tissue sections. We tested four histologically confirmed cases of SBC for the presence of the <jats:italic>ETV6\u2010NTRK3<\/jats:italic> gene fusion and then applied the FISH assay to tissue microarrays (TMAs) in order to screen 481 cases of formalin\u2010fixed, paraffin\u2010embedded invasive breast carcinomas of various histologic subtypes. Three of the four cases of SBC revealed fusion signals. Of the 481 cases in the TMAs, 202 gave signals of sufficient quality for screening by FISH, and only one case showed fusion signals in most or all of the tumor cells. On review of the histology of this case, SBC was confirmed. On the other hand, none of the fusion\u2010negative breast cancers revealed SBC histology. In all cases, the results from the fusion and split\u2010apart FISH assays for the <jats:italic>ETV6\u2010NTRK3<\/jats:italic> fusion genes were concordant. Our data suggest that the <jats:italic>ETV6\u2010NTRK3<\/jats:italic> fusion gene is a specific genetic alteration in SBC. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20028","type":"journal-article","created":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T21:02:52Z","timestamp":1080334972000},"page":"152-157","source":"Crossref","is-referenced-by-count":121,"title":["A fluorescence in situ hybridization study of <i>ETV6\u2010NTRK3<\/i> fusion gene in secretory breast carcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"Nikita","family":"Makretsov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"May","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm","family":"Hayes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Chia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doug E.","family":"Horsman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poul H. 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