{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:24:39Z","timestamp":1780629879269,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,12,20]],"date-time":"2004-12-20T00:00:00Z","timestamp":1103500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006280","name":"Spanish Ministry of Science and Technology","doi-asserted-by":"crossref","award":["SAF-01-0056"],"award-info":[{"award-number":["SAF-01-0056"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Red Tem\u00e1tica de Centros del C\u00e1ncer, Spanish Ministry of Health","award":["CO-010"],"award-info":[{"award-number":["CO-010"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molecular cytogenetic techniques enabled us to clarify numerical and structural alterations previously detected by conventional cytogenetic techniques in 37 patients who had myelodysplastic syndromes with complex karyotypes. Using high\u2010resolution comparative genomic hybridization (HR\u2010CGH), we found the most recurrent alterations to be deletion of 5q (70%), 18q (35%), 7q (32%), 11q (30%), and 20q (24%), gain of 11q (35%) and 8q (24%), and trisomy of chromosome 8 (19%). Furthermore, in 35% of the patients, 20 amplifications were identified. These amplifications were shown by FISH to involve some genes previously described as amplified in hematological malignancies, such as <jats:italic>ERBB2<\/jats:italic>, <jats:italic>MLL<\/jats:italic>, and <jats:italic>RUNX1<\/jats:italic>. In addition, two other genes, <jats:italic>BCL6<\/jats:italic> and <jats:italic>BCL2<\/jats:italic>, which are classically related to apoptosis and non\u2010Hodgkin lymphoma, were shown for the first time to be involved in amplification. Genomic alterations involving different subtelomeric regions with losses in 4p16, 5p15.3, 6q27, 18p11.3, and 18q23 and gains in 1p36.3 and 19p13.3 were detected by HR\u2010CGH. Array CGH analysis of the subtelomeric regions in some samples was able to confirm a number of these alterations and found some additional alterations not detected by conventional CGH. \u00a92004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20154","type":"journal-article","created":{"date-parts":[[2004,12,20]],"date-time":"2004-12-20T22:32:55Z","timestamp":1103581975000},"page":"287-298","source":"Crossref","is-referenced-by-count":38,"title":["Analysis of myelodysplastic syndromes with complex karyotypes by high\u2010resolution comparative genomic hybridization and subtelomeric CGH array"],"prefix":"10.1002","volume":"42","author":[{"given":"Angel","family":"Mart\u00ednez\u2010Ram\u00edrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Urioste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Melchor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Blesa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Valle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Alvarez","family":"de Andr\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaas","family":"Kok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jos\u00e9","family":"Calasanz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Cruz","family":"Cigudosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Ben\u00edtez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,12,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64897-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.042702599"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1984.tb03982.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10180"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401919"},{"key":"e_1_2_6_7_1","first-page":"S19","article-title":"Cytogenetic and molecular cytogenetic analysis of B cell chronic lymphocytic leukemia: specific chromosome aberrations identify prognostic subgroups of patients and point to loci of candidate genes","volume":"11","author":"Dohner H","year":"1997","journal-title":"Leukemia"},{"key":"e_1_2_6_8_1","first-page":"127","article-title":"Cytogenetics of myelodysplastic syndromes","volume":"33","author":"Fenaux P","year":"1996","journal-title":"Semin Hematol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10628"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10155"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.6.2079"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.7.2322"},{"key":"e_1_2_6_13_1","volume-title":"Cancer Cytogenetics. Chromosomal and molecular genetic aberrations of tumor cells","author":"Heim S","year":"1995"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02723.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199912)26:4<336::AID-GCC8>3.0.CO;2-H"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1359641"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(00)00386-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980301)31:3<163::AID-CYTO3>3.0.CO;2-M"},{"key":"e_1_2_6_19_1","first-page":"1158","article-title":"Molecular cytogenetic characterization of acute myeloid leukemia and myelodysplastic syndromes with multiple chromosome rearrangements","volume":"86","author":"Lindvall C","year":"2001","journal-title":"Haematologica"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2003.12.014"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2264(2000)9999:9999<::AID-GCC1003>3.0.CO;2-U"},{"key":"e_1_2_6_22_1","volume-title":"Supplement to an International System for Human Cytogenetic Nomenclature","author":"Mitelman F","year":"1995"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10027"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-06-2163"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.32.1.495"},{"key":"e_1_2_6_26_1","first-page":"59","article-title":"The role of chromosome translocations in leukemogenesis","volume":"36","author":"Rowley JD","year":"1999","journal-title":"Semin Hematol"},{"key":"e_1_2_6_27_1","volume-title":"The chromosomes in human cancer and leukemia","author":"Sandberg AA","year":"1990"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10088"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(200004)27:4<380::AID-GCC7>3.0.CO;2-#"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.1212"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(00)00287-9"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00311.x"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20154","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T22:11:47Z","timestamp":1700172707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,20]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1002\/gcc.20154"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20154","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,20]]}}}