{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:46:29Z","timestamp":1791179189401,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,4,15]],"date-time":"2005-04-15T00:00:00Z","timestamp":1113523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>AGR2<\/jats:italic>\n                    , the human homologue of\n                    <jats:italic>Xenopus anterior gradient 2 (XAG2),<\/jats:italic>\n                    was identified by a suppression subtractive hybridization\u2010based technique as an androgen\u2010inducible gene. There are two\n                    <jats:italic>AGR2<\/jats:italic>\n                    transcripts, which encode the same secretory protein of 175 amino acids. The androgen induction was time\u2010 and dose\u2010dependent, with more than a 10\u2010fold increase in the level of\n                    <jats:italic>AGR2<\/jats:italic>\n                    mRNA after 48 hr of treatment with 10\n                    <jats:sup>\u22129<\/jats:sup>\n                    M R1881. Expression of\n                    <jats:italic>AGR2<\/jats:italic>\n                    mRNA was specifically detected in limited human tissue rich in epithelial cells, including the prostate gland. Analysis of 46 microdissected primary prostate adenocarcinoma samples showed that\n                    <jats:italic>AGR2<\/jats:italic>\n                    mRNA expression was markedly elevated in the majority of tumors as compared to matched adjacent benign tissues. Androgen\u2010induced AGR2 protein expression was demonstrated in LNCaP cells by Western blot analysis with an anti\u2010AGR2 antibody. Immunohistochemistry analysis indicated that AGR2 protein expression was highly restricted to the secretory epithelial cells in the prostate gland. In tissue sections from radical prostatectomy specimens, immunohistochemical staining of AGR2 showed markedly increased expression in high\u2010grade prostatic intraepithelial neoplasia and Gleason pattern 3\u22124 prostatic adenocarcinoma. Therefore, the androgen\u2010induced secretory protein AGR2 may serve as a potential therapeutic target and\/or molecular marker for prostate cancer. \u00a9 2005 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/gcc.20188","type":"journal-article","created":{"date-parts":[[2005,4,15]],"date-time":"2005-04-15T14:47:07Z","timestamp":1113576427000},"page":"249-259","source":"Crossref","is-referenced-by-count":122,"title":["AGR2, an androgen\u2010inducible secretory protein overexpressed in prostate cancer"],"prefix":"10.1002","volume":"43","author":[{"given":"Jin\u2010San","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiyu","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Cheville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David I.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Y. F.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(98)00021-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.6.1215"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2388(200001\/02)18:1<3::AID-SSU2>3.0.CO;2-I"},{"key":"e_1_2_6_5_1","first-page":"995","article-title":"Generation and genetic characterization of immortal human prostate epithelial cell lines derived from primary cancer specimens","volume":"57","author":"Bright RK","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/6495"},{"key":"e_1_2_6_7_1","first-page":"1095","article-title":"The androgen receptor in hormone\u2010refractory prostate cancer: relevance of different mechanisms of androgen receptor signaling","volume":"23","author":"Cronauer MV","year":"2003","journal-title":"Int J Oncol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000075099.20662.7f"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.1073\/pnas.93.12.6025","article-title":"Suppression subtractive hybridization: a method for generating differentially regulated or tissue\u2010specific cDNA","volume":"93","author":"Diatchenko L","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35094009"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600740"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0094-0143(03)00057-0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.54.1.8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(99)00010-X"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(02)00064-X"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10653"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9440"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00845-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80067-1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.15.5041-5049.2001"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5458"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2264(2000)9999:9999<::AID-GCC1070>3.0.CO;2-W"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20188","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T12:43:12Z","timestamp":1697460192000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,15]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1002\/gcc.20188"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20188","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,15]]}}}