{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:01:55Z","timestamp":1791158515084,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,7,6]],"date-time":"2005-07-06T00:00:00Z","timestamp":1120608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To delineate multiple myeloma (MM) subgroups and their clonal evolution, we analyzed 81 newly diagnosed patients by interphase fluorescence in situ hybridization using a comprehensive probe set for 10 chromosomes and two <jats:italic>IGH<\/jats:italic> rearrangements. A median of 5 probes per patient displayed aberrant signal numbers (range, 1\u201310). Additional copies most frequently found were for 15q22, 19q13, 9q34, 11q23, and 1q21. Losses commonly observed were of 13q14.3, 17p13, and 22q11. Predominance of gain or loss was quantified by a copy number score (CS) for each patient. Two peaks (CS = +3 and CS = 0) were found by plotting patient copy number scores over CS values corresponding to hyperdiploid and nonhyperdiploid MM. Cluster analysis revealed four major branches: (i) gain of 9q, 15q, 19q, and\/or 11q; (ii) deletion of 13q and t(4;14); (iii) t(11;14); and (iv) gain of 1q. Statistical modeling of an oncogenetic tree indicated that early independent events were gain of 15q\/9q and\/or 11q, t(11;14); deletion of 13q followed by t(4;14); and gain of 1q. Aberrations of 17p13, 22q11, 8p12, and 6q21 were found as subsequent events. MM with gain of 1q was delineated as a subentity with significantly higher beta\u20102\u2010microglobulin and lower hemoglobin levels, indicating a poor prognosis. From our results, we propose a model of MM for clonal evolution. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20231","type":"journal-article","created":{"date-parts":[[2005,7,6]],"date-time":"2005-07-06T23:16:53Z","timestamp":1120691813000},"page":"194-203","source":"Crossref","is-referenced-by-count":74,"title":["Delineation of distinct subgroups of multiple myeloma and a model for clonal evolution based on interphase cytogenetics"],"prefix":"10.1002","volume":"44","author":[{"given":"Friedrich W.","family":"Cremer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Bila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Buck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutlu","family":"Kartal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Hose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Ittrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Benner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc S.","family":"Raab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann\u2010Cathrin","family":"Theil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Moos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Goldschmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus R.","family":"Bartram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Jauch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,7,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00052.x"},{"key":"e_1_2_6_3_1","first-page":"533","article-title":"Prognostic factors in multiple myeloma: It's in the genes","volume":"9","author":"Bergsagel PL","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1182\/blood.V91.8.3007.3007_3007_3010","article-title":"Characterization of nonrandom chromosomal gains and losses in multiple myeloma by comparative genomic hybridization","volume":"91","author":"Cigudosa JC","year":"1998","journal-title":"Blood"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-01-0016"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402797"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/10665270050514936"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.3.802"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb03155.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03757.x"},{"key":"e_1_2_6_11_1","first-page":"300","article-title":"Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells","volume":"49","author":"Fiserova A","year":"2002","journal-title":"Neoplasma"},{"key":"e_1_2_6_12_1","first-page":"715","article-title":"Biological and prognostic significance of interphase fluorescence in situ hybridization detection of chromosome 13 abnormalities in multiple myeloma: An Eastern Cooperative Oncology Group Study","volume":"62","author":"Fonseca R","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-02-0493"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-2876"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428199909145707"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402116"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1319"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1182\/blood.V91.1.3","article-title":"Multiple myeloma: Increasing evidence for a multistep transformation process","volume":"9","author":"Hallek M","year":"1998","journal-title":"Blood"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00951.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-01-0037"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10314"},{"key":"e_1_2_6_22_1","volume-title":"Nonparametric statistical methods","author":"Hollander M","year":"1973"},{"key":"e_1_2_6_23_1","first-page":"65","article-title":"A simple sequentially rejective multiple test procedure","volume":"6","author":"Holm S","year":"1979","journal-title":"Scandinavian Journal of Statistics"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/4W8E-8F4K-BHUP-UBE7"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.2307\/1390807"},{"key":"e_1_2_6_26_1","volume-title":"An international system for human cytogenetic nomenclature","author":"ISCN.","year":"1995"},{"key":"e_1_2_6_27_1","first-page":"175","article-title":"Multiple myeloma: evolving genetic events and host interactions","volume":"2","author":"Kuehl WM","year":"2002","journal-title":"Nature"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403153"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04417.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/001316446602600402"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910180105"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0749"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.959840"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00420933"},{"key":"e_1_2_6_35_1","first-page":"2539","article-title":"p53 and RAS gene mutations in multiple myeloma","volume":"7","author":"Portier M","year":"1992","journal-title":"Oncogene"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/mp.55.5.273"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb08253.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(99)00009-6"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.39.12.900"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403256"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.5.1732"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02546.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0889"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(03)01195-1"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2873"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.7.2229"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10275"},{"key":"e_1_2_6_48_1","first-page":"1409","article-title":"A statistical method for evaluating systematic relationships","volume":"38","author":"Sokal RR","year":"1958","journal-title":"University of Kansas Sci Bull"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-12-3789"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-3837"},{"key":"e_1_2_6_51_1","first-page":"153","article-title":"Incidence of chromosome numerical changes in multiple myeloma: fluorescence in situ hybridization analysis using 15 chromosome\u2010specific probes","volume":"149","author":"Tabernero D","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/kxh007"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403586"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-5710(95)00394-8"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(03)00197-5"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20231","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T09:12:57Z","timestamp":1697447577000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,6]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1002\/gcc.20231"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20231","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7,6]]}}}