{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T20:14:41Z","timestamp":1776716081766,"version":"3.51.2"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T00:00:00Z","timestamp":1123027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The loss of the Y chromosome is a frequent numerical chromosomal abnormality observed in human prostate cancer. In cancer, loss of specific genetic material frequently accompanies simultaneous inactivation of tumor suppressor genes. It is not known whether the Y chromosome harbors such genes. To address the role of genes on the Y chromosome in human prostate cancer, we transferred a tagged Y chromosome into PC\u20103, a human prostate cancer cell line lacking a Y chromosome. A human Y chromosome was tagged with the <jats:italic>hisD<\/jats:italic> gene and transferred to PC\u20103 by microcell\u2010mediated chromosome transfer. Tumorigenicity of these PC\u20103 hybrids was tested in vivo and in vitro, and the results were compared with those of the polymerase chain reaction analyses conducted on the PC\u20103 hybrids using Y chromosome\u2010specific markers. Among 60 mice injected with 12 different PC\u20103 hybrids (five mice per hybrid), tumor growth was apparent in only one mouse, whereas tumors grew in all mice injected with the parental PC\u20103 cells. An in vitro assay showed that the Y chromosome did not suppress anchorage\u2010independent growth of PC\u20103 cells. We found that addition of the Y chromosome suppressed tumor formation by PC\u20103 in athymic nude mice, and that this block of tumorigenesis was independent of the in vitro growth properties of the cells. This observation suggests the presence of a gene important for prostate tumorigenesis on the Y chromosome. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20250","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T21:28:34Z","timestamp":1123104514000},"page":"365-372","source":"Crossref","is-referenced-by-count":40,"title":["The human Y chromosome suppresses the tumorigenicity of PC\u20103, a human prostate cancer cell line, in athymic nude mice"],"prefix":"10.1002","volume":"44","author":[{"given":"Sapna","family":"Vijayakumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuck 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