{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:45:20Z","timestamp":1791283520048,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,28]],"date-time":"2005-12-28T00:00:00Z","timestamp":1135728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009729","name":"Ludwig Institute for Cancer Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009729","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000884","name":"Cancer Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000884","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cancer\/testis (CT) genes are normally expressed in germ cells only, yet are reactivated and expressed in some tumors. Of the approximately 40 CT genes or gene families identified to date, 20 are on the X chromosome and are present as multigene families, many with highly conserved members. This indicates that novel CT gene families may be identified by detecting duplicated expressed genes on chromosome X. By searching for transcript clusters that map to multiple locations on the chromosome, followed by in silico analysis of their gene expression profiles, we identified five novel gene families with testis\u2010specific expression and &gt;98% sequence identity among family members. The expression of these genes in normal tissues and various tumor cell lines and specimens was evaluated by qualitative and quantitative RT\u2010PCR, and a novel CT gene family with at least 13 copies was identified on Xq24, designated as <jats:italic>CT47<\/jats:italic>. mRNA expression of <jats:italic>CT47<\/jats:italic> was found mainly in the testes, with weak expression in the placenta. Brain tissue was the only positive somatic tissue tested, with an estimated <jats:italic>CT47<\/jats:italic> transcript level 0.09% of that found in testis. Among the tumor specimens tested, <jats:italic>CT47<\/jats:italic> expression was found in \u223c15% of lung cancer and esophageal cancer specimens, but not in colorectal cancer or breast cancer. The putative CT47 protein consists of 288 amino acid residues, with a C\u2010terminus rich in alanine and glutamic acid. The only species other than human in which a gene homologous to <jats:italic>CT47<\/jats:italic> has been detected is the chimpanzee, with the predicted protein showing \u223c80% identity in its carboxy terminal region. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20298","type":"journal-article","created":{"date-parts":[[2005,12,28]],"date-time":"2005-12-28T20:16:40Z","timestamp":1135801000000},"page":"392-400","source":"Crossref","is-referenced-by-count":34,"title":["Identification of a new cancer\/testis gene family, <i>CT47<\/i>, among expressed multicopy genes on the human X chromosome"],"prefix":"10.1002","volume":"45","author":[{"given":"Yao\u2010Tseng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Iseli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charis A.","family":"Venditti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd J.","family":"Old","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J. G.","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Victor","family":"Jongeneel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-004-2654-3"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1860604"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.725"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502583102"},{"key":"e_1_2_5_6_1","first-page":"5544","article-title":"An overview of the MAGE gene family with the identification of all human members of the family","volume":"61","author":"Chomez P","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_5_7_1","first-page":"3157","article-title":"Characterization of the GAGE genes that are expressed in various human cancers and in normal testis","volume":"59","author":"De Backer O","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601062"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.9.967"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000301)85:5<726::AID-IJC21>3.0.CO;2-F"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10634"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.62002"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000701)87:1<55::AID-IJC8>3.0.CO;2-J"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03440"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.25.11810"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10276"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2002.18803.x"},{"key":"e_1_2_5_18_1","first-page":"1","article-title":"The cancer\/testis genes: review, standardization, and commentary","volume":"4","author":"Scanlan MJ","year":"2004","journal-title":"Cancer Immunity"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1864804"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242603899"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2542904"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/10665270050081478"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20298","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T15:05:21Z","timestamp":1694444721000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,28]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1002\/gcc.20298"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20298","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,28]]}}}