{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:52:53Z","timestamp":1761310373064},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T00:00:00Z","timestamp":1157500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The early and frequent occurrence of deletions at 3p21.3 in lung cancer has led to the consideration of this chromosomal region as a lung cancer (LUCA) critical region with tumor suppressor activity. We covered this 19 genes\u2010containing region with overlapping P1 artificial chromosomes (PACs), in which genes are likely accompanied by their own promoters or other regulatory sequences. With these PACs we transfected cells from a small cell lung cancer (SCLC) cell line which readily caused tumors in nude mice. Per PAC we selected two cell clones with a low number of PAC copies integrated at a single genomic site. The selected clones were s.c. injected into nude mice to investigate whether the integrated genes suppressed the tumor\u2010inducing capacity of the original SCLC cell line. We could demonstrate PAC\u2010specific gene expression in the transfected cells. All of the PAC integration sites were different. It appeared that introduction of a PAC or even an empty PAC vector causes some chromosomal instability, which in principle may either promote or inhibit cell growth. However, both cell clones with integration of the same PAC from the centromeric part of the LUCA region in different genomic sites were the sole pair of clones that caused smaller tumors than did the original SCLC cell line. This suggests that rather than the induced chromosomal instability, the DNA sequence of that PAC, which in addition to two protein\u2010encoding genes contains at least one potential miRNA gene, is responsible for the tumor suppressor activity. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20367","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T20:51:51Z","timestamp":1157575911000},"page":"1077-1093","source":"Crossref","is-referenced-by-count":8,"title":["Functional analysis of lung tumor suppressor activity at 3p21.3"],"prefix":"10.1002","volume":"45","author":[{"given":"Arja","family":"ter Elst","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bea E.","family":"Hiemstra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter","family":"van der Vlies","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wytske","family":"Kamminga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke Y.","family":"van der Veen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge","family":"Davelaar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Terpstra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard J.","family":"te Meerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans","family":"Gerbens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaas","family":"Kok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles H. C. M.","family":"Buys","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204175"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3672"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.12.031"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.8.971"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.9.691"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-85"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20079"},{"key":"e_1_2_7_9_1","first-page":"1721","article-title":"A homozygous deletion on chromosome 3 in a small cell lung cancer cell line correlates with a region of tumor suppressor activity","volume":"8","author":"Daly MC","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204469"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131216298"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10155"},{"key":"e_1_2_7_13_1","first-page":"1631","article-title":"Aurora\u2010A\/STK15\/BTAK enhances chromosomal instability in bladder cancer cells","volume":"25","author":"Fraizer GC","year":"2004","journal-title":"Int J Oncol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6286"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0898-6568(95)02049-7"},{"key":"e_1_2_7_16_1","first-page":"2715","article-title":"Expression of several genes in the human chromosome 3p21.3 homozygous deletion region by an adenovirus vector results in tumor suppressor activities in vitro and in vivo","volume":"62","author":"Ji L","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208659"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60096-2"},{"key":"e_1_2_7_19_1","first-page":"4183","article-title":"A homozygous deletion in a small cell lung cancer cell line involving a 3p21 region with a marked instability in yeast artificial chromosomes","volume":"54","author":"Kok K","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2146903"},{"key":"e_1_2_7_21_1","first-page":"6116","article-title":"The 630\u2010kb lung cancer homozygous deletion region on human chromosome 3p21.3: Identification and evaluation of the resident candidate tumor suppressor genes","volume":"60","author":"Lerman MI","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207789"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11166"},{"key":"e_1_2_7_24_1","first-page":"3207","article-title":"A candidate tumor suppressor gene, H37, from the human lung cancer tumor suppressor locus 3p21.3","volume":"62","author":"Oh JJ","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.10.2887"},{"key":"e_1_2_7_26_1","first-page":"1289","article-title":"Distinct 3p21.3 deletions in lung cancer and identification of a new human semaphorin","volume":"12","author":"Roche J","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2085"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3613"},{"key":"e_1_2_7_29_1","first-page":"61","article-title":"DNA methylation profiles of lung tumors","volume":"1","author":"Toyooka S","year":"2001","journal-title":"Mol Cancer Ther"},{"key":"e_1_2_7_30_1","first-page":"542","article-title":"Human Semaphorin 3B (SEMA3B) located at chromosome 3p21.3 suppresses tumorformation in an adenocarcinoma cell line","volume":"62","author":"Tse C","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(89)90158-1"},{"key":"e_1_2_7_32_1","first-page":"1487","article-title":"Construction of a 600\u2010kilobase cosmid clone contig and generation of a transcriptional map surrounding the lung cancer tumor suppressor gene (TSG) locus on human chromosome 3p21.3: Progress toward the isolation of a lung cancer TSG","volume":"56","author":"Wei MH","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.18.1366"},{"key":"e_1_2_7_34_1","first-page":"2637","article-title":"Semaphorin 3F gene from human 3p21.3 suppresses tumor formation in nude mice","volume":"62","author":"Xiang R","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205835"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(82)90077-2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6274023"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20367","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T18:26:23Z","timestamp":1697480783000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,6]]},"references-count":36,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1002\/gcc.20367"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20367","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,6]]}}}