{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:44:52Z","timestamp":1790315092456,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:00:00Z","timestamp":1171929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The t(1;19)(q23;p13.3) is one of the most common chromosomal abnormalities in B\u2010cell precursor acute lymphoblastic leukemia (BCP\u2010ALL) and usually gives rise to the <jats:italic>TCF3<\/jats:italic>\u2010<jats:italic>PBX1<\/jats:italic> fusion gene. Additional rare, and sometimes cytogenetically cryptic, translocations involving the <jats:italic>TCF3<\/jats:italic> gene have also been described. Using a dual color split\u2010signal fluorescence in situ hybridization (FISH) probe, we have investigated the involvement of this gene in a series of BCP\u2010ALLs harboring 19p13 translocations, as well as an unselected patient cohort. The <jats:italic>TCF3<\/jats:italic> gene was shown to be involved in the majority of cases with a cytogenetically visible t(1;19) translocation, while the remaining <jats:italic>TCF3<\/jats:italic>\u2010negative ALLs demonstrated breakpoint heterogeneity. Although most \u201cother\u201d 19p13 translocations did not produce a split\u2010signal FISH pattern, a novel t(13;19)(q14;p13) involving <jats:italic>TCF3<\/jats:italic> was discovered. A prospective screen of 161 children with BCP\u2010ALL revealed a cryptic t(12;19)(p13;p13), another novel <jats:italic>TCF3<\/jats:italic> rearrangement, and a series of patients with submicroscopic deletions of <jats:italic>TCF3<\/jats:italic>. These results demonstrate the utility of a split\u2010signal FISH strategy in confirming the involvement of the <jats:italic>TCF3<\/jats:italic> gene in 19p13 rearrangements and in identifying novel and cryptic <jats:italic>TCF3<\/jats:italic> translocations. In addition to its role as a fusion partner gene, we propose that <jats:italic>TCF3<\/jats:italic> can also act as a tumor suppressor gene in BCP\u2010ALL. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20431","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T21:29:06Z","timestamp":1172006946000},"page":"478-486","source":"Crossref","is-referenced-by-count":58,"title":["Molecular cytogenetic characterization of <i>TCF3 (E2A)<\/i>\/19p13.3 rearrangements in B\u2010cell precursor acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"46","author":[{"given":"Kerry E.","family":"Barber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine J.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe J.","family":"Broadfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam R. M.","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Martineau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon C.","family":"Strefford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony 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