{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:29:05Z","timestamp":1791196145881,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T00:00:00Z","timestamp":1175126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lysyl oxidase\u2010like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. We previously demonstrated the association between increased <jats:italic>LOXL2<\/jats:italic> expression and invasive\/metastatic behavior in human breast cancer cells and mouse squamous and spindle cell carcinomas, interaction between LOXL2 and SNAIL in epithelial\u2010mesenchymal transition, and localization of the <jats:italic>LOXL2<\/jats:italic> gene to 8p21.2\u201321.3, within a minimally deleted region in several cancers, including colon and esophagus. In the present study, we analyzed LOXL2 expression in colon and esophageal tumors, and explored methylation as a regulator of <jats:italic>LOXL2<\/jats:italic> expression. Immunohistochemistry using normal tissues demonstrated intracellular localization of LOXL2 in colonic enteroendocrine cells and esophageal squamous cells at the luminal surface, but not in mitotically active cells. Tissue array analysis of 52 colon adenocarcinomas and 50 esophageal squamous cell carcinomas revealed presence of LOXL2 expression in 83 and 92% of the samples, respectively, and a significant association between increased number of LOXL2\u2010expressing cells and less\u2010differentiated colon carcinomas. We determined that the methylation status of the 1150 bp 5\u2032 CpG island may contribute to the regulation of the gene. Loss of heterozygosity studies, using a microsatellite within intron 4 of the <jats:italic>LOXL2<\/jats:italic> gene, revealed that loss of <jats:italic>LOXL2<\/jats:italic> was unlikely to play a major role in either colon or esophageal tumors. These results suggest that increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20444","type":"journal-article","created":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T15:48:16Z","timestamp":1175183296000},"page":"644-655","source":"Crossref","is-referenced-by-count":101,"title":["Lysyl oxidase\u2010like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors"],"prefix":"10.1002","volume":"46","author":[{"given":"Sheri F. T.","family":"Fong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Dietzsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith S. 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