{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:45:37Z","timestamp":1791049537329,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T00:00:00Z","timestamp":1202860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Homozygous deletions in cancer cells have been thought to harbor tumor suppressor genes. We show that the 25 and 50 kb homozygous deletions in <jats:italic>WWOX<\/jats:italic> in the colon cancer cell line HCT116 result from a complex set of heterozygous deletions, some of which overlap to give homozygous loss. One of the heterozygous deletions has removed exons 6\u20138 of one allele of <jats:italic>WWOX<\/jats:italic>, and there is also a third copy of the distal region of <jats:italic>WWOX<\/jats:italic> in an unbalanced translocation. The exon 6\u20138 deletion results in allele\u2010specific expression of a deleted transcript, which seems likely to be the main biological consequence of the deletions, since similar transcripts are found in other tumors. We show that such a complex set of deletions could form in a single exchange event between two homologous chromosomes, so that the selective advantage of such rearrangements need not be within the homozygous deletion. We conclude that homozygous deletions can be markers of complex rearrangements that have targets outside the homozygous deletion itself and that the target of deletions in the <jats:italic>FRA16D<\/jats:italic> region is indeed <jats:italic>WWOX<\/jats:italic>, the common outcome being the removal of particular <jats:italic>WWOX<\/jats:italic> exons. This article contains supplementary material available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.interscience.wiley.com\/jpages\/1045-2257\/suppmat\">http:\/\/www.interscience.wiley.com\/jpages\/1045\u20102257\/suppmat<\/jats:ext-link>. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20548","type":"journal-article","created":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T16:23:06Z","timestamp":1202919786000},"page":"437-447","source":"Crossref","is-referenced-by-count":18,"title":["Homozygous deletions may be markers of nearby heterozygous mutations: The complex deletion at <i>FRA16D<\/i> in the HCT116 colon cancer cell line removes exons of <i>WWOX<\/i>"],"prefix":"10.1002","volume":"47","author":[{"given":"Amber E.","family":"Alsop","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieqing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Gabra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J. 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