{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:37:51Z","timestamp":1791589071301,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,3,3]],"date-time":"2008-03-03T00:00:00Z","timestamp":1204502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amplification at the 11q13 locus is commonly observed in breast, ovarian, head and neck, oral, and esophageal cancer. Studies of this region led to the identification of multiple amplicons containing several potential oncogenes including <jats:italic>EMSY<\/jats:italic>, <jats:italic>PAK1<\/jats:italic>, <jats:italic>RSF1<\/jats:italic>, and <jats:italic>GAB2<\/jats:italic>. Here, we investigate the amplification of the above four genes and their prognostic significance in histologically and clinically defined subsets of ovarian cancer. Amplification of all four genes was assessed by fluorescent in situ hybridization in tissue microarrays containing 538 clinically annotated ovarian carcinomas with 12 years of follow\u2010up data. Overall, for the entire cohort, <jats:italic>EMSY<\/jats:italic> was amplified in 44 (16%) of 269 cases, <jats:italic>PAK1<\/jats:italic> was amplified in 38 (15%) of 255 cases, <jats:italic>RSF1<\/jats:italic> was amplified in 37 (12%) of 310 cases, and <jats:italic>GAB2<\/jats:italic> was amplified in 41 (16%) of 255 cases. Amplification of <jats:italic>EMSY<\/jats:italic>, <jats:italic>PAK1<\/jats:italic>, <jats:italic>RSF1<\/jats:italic>, and <jats:italic>GAB2<\/jats:italic> were all highly correlated with each other and with a serous histology. Univariate survival analysis showed that tumors with <jats:italic>EMSY<\/jats:italic> and <jats:italic>RSF1<\/jats:italic> amplification were associated with a significantly worse outcome. A molecular inversion probe array was then used to study the 11q13 amplicon in 33 high grade serous carcinomas. The core of the amplicon mapped to a 6\u2010Mb region encompassing <jats:italic>EMSY<\/jats:italic>, <jats:italic>PAK1<\/jats:italic>, <jats:italic>RSF1<\/jats:italic>, and <jats:italic>GAB2<\/jats:italic>. However, a second more telomeric amplicon was also observed for which no candidate genes have been identified. In summary, amplification of these four putative oncogenes from 11q13 in early ovarian cancer is associated with a serous histology and in the case of <jats:italic>EMSY<\/jats:italic> and <jats:italic>RSF1<\/jats:italic> a poor outcome. These findings support the hypothesis that the11q13 amplicon in ovarian cancer is likely driven by a cassette of genes rather than by a single oncogene. This article contains Supplementary Material available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.interscience.wiley.com\/jpages\/1045-2257\/suppmat\">http:\/\/www.interscience.wiley.com\/jpages\/1045\u20102257\/suppmat<\/jats:ext-link>. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20549","type":"journal-article","created":{"date-parts":[[2008,3,3]],"date-time":"2008-03-03T23:16:26Z","timestamp":1204586186000},"page":"481-489","source":"Crossref","is-referenced-by-count":107,"title":["Amplification of 11q13 in ovarian carcinoma"],"prefix":"10.1002","volume":"47","author":[{"given":"Lindsay A.","family":"Brown","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve E.","family":"Kalloger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda A.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ie\u2010Ming","family":"Shih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.","family":"McKinney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Swenerton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Spellman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Gray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Blake","family":"Gilks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Huntsman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,3,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2006.06.007"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000134699"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1341"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.08.026"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","DOI":"10.2202\/1544-6115.1095","article-title":"Statistical analysis of adsorption models for oligonucleotide microarrays","volume":"3","author":"Burden CJ","year":"2004","journal-title":"Stat Appl Genet Mol Biol"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960822)69:4<247::AID-IJC1>3.0.CO;2-X"},{"key":"e_1_2_5_8_1","first-page":"4360","article-title":"Mapping of DNA amplifications at 15 chromosomal localizations in 1875 breast tumors: Definition of phenotypic groups","volume":"57","author":"Courjal F","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-96"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-006-0299-6"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3185605"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172285799"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20371"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00930-9"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202023"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209690"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870090108"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2006.04.008"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023033708204"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji254"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0410"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63458-X"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00562-7"},{"key":"e_1_2_5_24_1","first-page":"5229","article-title":"Amplification of genes within the chromosome 11q13 region is indicative of poor prognosis in patients with operable breast cancer","volume":"52","author":"Schuuring E","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199806)20:6<473::AID-BIES5>3.0.CO;2-N"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111354200"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2002.6717"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504195102"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002138200"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.6.1899-1910.2000"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni177"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204322"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20549","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20549","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T18:56:20Z","timestamp":1699988180000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,3]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1002\/gcc.20549"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20549","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,3]]}}}