{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:57:08Z","timestamp":1761292628129},"reference-count":67,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T00:00:00Z","timestamp":1244678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, the concept of cancer stem cells and their expression of embryonic stem cell markers has gained considerable experimental support. In this study, we examined the expression of one such marker, the <jats:italic>high\u2010mobility group AT\u2010hook 2 gene (HMGA2)<\/jats:italic> mRNA, in 53 formalin\u2010fixed, paraffin\u2010embedded mucoepidermoid carcinomas (MEC) and four normal parotid tissues using quantitative real\u2010time RT\u2010PCR (qPCR). MECs are often characterized by the fusion gene <jats:italic>CRTC1\u2010MAML2,<\/jats:italic> the detection of which is an important tool for the diagnosis and prognosis of MEC. For detection of the <jats:italic>CRTC1\u2010MAML2<\/jats:italic> fusion transcript, we performed RT\u2010PCR. The mean expression level of <jats:italic>HMGA2<\/jats:italic> was higher in fusion negative (302.8 \u00b1 124.4; <jats:italic>n<\/jats:italic> = 14) than in positive tumors (67.3 \u00b1 13.1; <jats:italic>n<\/jats:italic> = 39). Furthermore, the fusion\u2010negative tumors were often high\u2010grade tumors and the <jats:italic>HMGA2<\/jats:italic> expression level rose with the tumor grade (low: 43.7 \u00b1 11.0, intermediate: 126.2 \u00b1 28.3, and high: 271.2 \u00b1 126.5). A significant difference was found in the <jats:italic>HMGA2<\/jats:italic> expression levels between the different grading groups (one\u2010way ANOVA, <jats:italic>P<\/jats:italic> = 0.04) and among the fusion\u2010negative and \u2010positive tumors (<jats:italic>t<\/jats:italic>\u2010test, <jats:italic>P<\/jats:italic> = 0.05), indicating that the expression level of <jats:italic>HMGA2<\/jats:italic> was closely linked to grading, the presence\/absence of the <jats:italic>CRTC1\u2010MAML2<\/jats:italic> fusion, and the tumor behavior of MECs. These findings offer further evidence for the theory that the MEC group comprises two subgroups: one group with the <jats:italic>CRTC1\u2010MAML2<\/jats:italic> fusion, which is a group with a moderate aggressiveness and prognosis, and the other group lacking that fusion corresponding to an increased stemness, and thus, higher aggressiveness and worse prognosis. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20682","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T14:51:21Z","timestamp":1244731881000},"page":"777-785","source":"Crossref","is-referenced-by-count":16,"title":["A link between the expression of the stem cell marker <i>HMGA2<\/i>, grading, and the fusion <i>CRTC1\u2010MAML2<\/i> in mucoepidermoid carcinoma"],"prefix":"10.1002","volume":"48","author":[{"given":"Andr\u00e9","family":"Fehr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anke","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Heidorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"R\u00f6ser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"L\u00f6ning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rn","family":"Bullerdiek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,6,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700303"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19920415)69:8<2021::AID-CNCR2820690803>3.0.CO;2-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20168"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20306"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20505"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20534"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.3.1545"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00123166"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90131-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(90)90250-E"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(93)90054-P"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)60360-8"},{"key":"e_1_2_6_14_1","first-page":"2439","article-title":"High level expression of the HMGI (Y) gene during embryonic development","volume":"13","author":"Chiappetta G","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2003.08.013"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1125"},{"key":"e_1_2_6_17_1","first-page":"202","article-title":"Clear cell hidradenoma of the skin\u2014A third tumor type with a t(11;19)\u2010associated TORC1\u2010MAML2 gene fusion","volume":"43","author":"El\u2010Naggar AK","year":"2006","journal-title":"Genes Chromosomes Cancer"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2003.09.007"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2007.10.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20522"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2007.02878.x"},{"key":"e_1_2_6_22_1","first-page":"13","article-title":"Expression of reciprocal hybrid transcripts of HMGIC and FHIT in a pleomorphic adenoma of the parotid gland","volume":"57","author":"Geurts JM","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201609"},{"key":"e_1_2_6_24_1","first-page":"219","volume-title":"World Health Organization Classification of Tumors. 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