{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:57:13Z","timestamp":1791043033171,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T00:00:00Z","timestamp":1245024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High\u2010throughput microarray technologies were used to study DNA methylation accompanied by transcriptional changes in follicular lymphoma (FL). Using Methylated CpG Island Amplification with Microarrays to study CpG Island DNA methylation in FL, we discovered widespread hypermethylation of homeobox genes and previously identified targets of polycomb repressive complex 2 (PRC2) in cell lines and primary tumors, but not in benign follicular hyperplasia (BFH). DNA methylation for <jats:italic>HOXA11<\/jats:italic>, <jats:italic>HOXD10<\/jats:italic>, <jats:italic>HOXB7<\/jats:italic>, <jats:italic>HOXC12<\/jats:italic>, <jats:italic>PAX6<\/jats:italic>, <jats:italic>LHX9<\/jats:italic>, <jats:italic>SFMBT2<\/jats:italic>, <jats:italic>EN2<\/jats:italic>, and <jats:italic>PAX7<\/jats:italic> was independently validated in the RL cell line and <jats:italic>HOXA11<\/jats:italic>, <jats:italic>HOXD10<\/jats:italic>, <jats:italic>PAX6<\/jats:italic>, and <jats:italic>EN2<\/jats:italic> in primary tumors. Combined Bisulfite Restriction Analysis (COBRA) also established DNA methylation for the previously identified PRC2 targets <jats:italic>DCC<\/jats:italic>, <jats:italic>DES<\/jats:italic>, <jats:italic>GAD2<\/jats:italic>, <jats:italic>AQP5<\/jats:italic>, <jats:italic>GPR61<\/jats:italic>, <jats:italic>GRIA4<\/jats:italic>, <jats:italic>GJD2<\/jats:italic>, and <jats:italic>AMPH<\/jats:italic> in FL but not in BFH. Gene expression analyses revealed 411 genes that were hypermethylated and transcriptionally repressed in RL, 74% of which were reactivated by the demethylating agent 5\u2010aza\u20102\u2032\u2010deoxycytidine (5\u2010azaD) plus or minus the histone deacetylase inhibitor trichostatin A (TSA). Forty genes were also downregulated in primary FL. Our results suggest that extensive hypermethylation in promoters of polycomb target genes is a characteristic of FL and that loss of expression of certain SUZ12 target genes could be functionally relevant for lymphomagenesis. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20687","type":"journal-article","created":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T19:58:22Z","timestamp":1245095902000},"page":"828-841","source":"Crossref","is-referenced-by-count":63,"title":["DNA hypermethylation accompanied by transcriptional repression in follicular lymphoma"],"prefix":"10.1002","volume":"48","author":[{"given":"Lynda B.","family":"Bennett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Schnabel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean 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