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In this study, we analyzed gene\u2010expression profiles of patients with previously untreated MM. Fifty\u2010four genes were significantly differentially expressed between the two groups. <jats:italic>NPM1<\/jats:italic> was upregulated in HD. The differential expression of 25 genes, including <jats:italic>NPM1<\/jats:italic> and 13 ribosomal protein genes, was validated using a published gene expression data set. The overexpression of <jats:italic>NPM1<\/jats:italic> in HD was further confirmed by quantitative real\u2010time PCR and Western blotting. <jats:italic>NPM1<\/jats:italic> was significantly overexpressed in HD as the result of a gain of chromosome 5. Insertions into exon 12 of <jats:italic>NPM1<\/jats:italic> were not detected. NPM1 was localized to the nucleoli of MM cells. Furthermore, HD was associated with an overexpression of ribosomal protein genes, independent of their localization on the trisomic or other chromosomes. Our results indicate that the gain of chromosome 5 might play an important role in the pathogenesis of HD. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20745","type":"journal-article","created":{"date-parts":[[2010,1,13]],"date-time":"2010-01-13T15:45:43Z","timestamp":1263397543000},"page":"333-341","source":"Crossref","is-referenced-by-count":9,"title":["NPM1 is overexpressed in hyperdiploid multiple myeloma due to a gain of chromosome 5 but is not delocalized to the cytoplasm"],"prefix":"10.1002","volume":"49","author":[{"given":"Niels","family":"Weinhold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerome","family":"Moreaux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc 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