{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:13:53Z","timestamp":1773360833943,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T00:00:00Z","timestamp":1275436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The chromosome location, 11q21\u201023, is linked to loss of heterozygosity (LOH) in multiple tumors including those of breast, lung, and head and neck. To examine the process of LOH induction, the H292 cell line (human muco\u2010epidermoid carcinoma) was irradiated or treated with anti\u2010CD95 antibody, and individual clones isolated through two rounds of cloning. Regions of LOH were determined by screening a suite of eight polymorphic microsatellite markers covering 11p15\u201011q24 using fluorescent primers and genetic analyzer peak discrimination. LOH induction was observed extending through 11q21.1\u201011q23.3 in 6\/49 of clones surviving 4 Gy and 8\/50 after 8 Gy. Analysis of selected clones by Affymetrix 6.0 single nucleotide polymorphism (SNP) arrays confirmed the initial assessment indicating a consistent 27.3\u201327.7 Mbp deletion in multiple clones. The telomeric border of LOH mapped to a 1 Mbp region of elevated recombination. Whole genome analysis of SNP data indicated that site\u2010restricted LOH also occurred across multiple additional genomic locations. These data indicate that 11q21.1\u201011q23.3, and potentially other regions of this cell line are sites of intrinsic cell\u2010specific instability leading to LOH after irradiation. Such deletions may subsequently be propagated by genetic selection and clonal expansion. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20791","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T17:58:09Z","timestamp":1275501489000},"page":"831-843","source":"Crossref","is-referenced-by-count":4,"title":["11q21.1\u201011q23.3 Is a site of intrinsic genomic instability triggered by irradiation"],"prefix":"10.1002","volume":"49","author":[{"given":"Nga","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela M.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"To Uyen","family":"Do","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Bien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol M.","family":"Bier\u2010Laning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheetal","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shyh\u2010Jen","family":"Shih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manual O.","family":"Diaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew T.","family":"Vaughan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2008.11.007"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10079"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20534"},{"key":"e_1_2_5_5_1","first-page":"5248","article-title":"A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: Development of international criteria for the determination of microsatellite instability in colorectal cancer","volume":"58","author":"Boland CR","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530000138826"},{"key":"e_1_2_5_7_1","first-page":"1727","article-title":"A genetic explanation of Slaughter's concept of field cancerization: Evidence and clinical implications","volume":"63","author":"Braakhuis BJ","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.6.1061"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1697"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2008.12.006"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20682"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2866"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00075.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02226.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2002.tb01329.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.026393"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000244472.56198.84"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307276101"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2007.05.013"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR1096.1"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng917"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2008.5"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn875"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/86934"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20435"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.25.120191.001433"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1366"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1934602100"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1097"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.8988"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02967591"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.113"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2006.12.004"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20462"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.117"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402237"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1011"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209624"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(195309)6:5<963::AID-CNCR2820060515>3.0.CO;2-Q"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.2.733-743.2003"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200403000-00006"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(02)00224-5"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2003.10.007"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200812"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.297.2.187"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600719"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20151"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.20791","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.20791","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T15:53:07Z","timestamp":1700581987000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.20791"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,2]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/gcc.20791"],"URL":"https:\/\/doi.org\/10.1002\/gcc.20791","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,2]]}}}