{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:44:54Z","timestamp":1791873894660,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,9,2]],"date-time":"2010-09-02T00:00:00Z","timestamp":1283385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diagnosis of myoepithelial (ME) tumors outside salivary glands remains challenging, especially in unusual clinical presentations, such as bone or visceral locations. A few reports have indicated <jats:italic>EWSR1<\/jats:italic> gene rearrangement in soft tissue ME tumors, and, in one case each, the fusion partner was identified as either <jats:italic>PBX1<\/jats:italic> or <jats:italic>ZNF444<\/jats:italic>. However, larger studies to investigate whether these genetic abnormalities are recurrent or restricted to tumors in soft tissue locations are lacking. Sixty\u2010six ME tumors mainly from soft tissue (71%), but also from skin, bone, and visceral locations, characterized by classic morphological features and supporting immunoprofile were studied. Gene rearrangements in <jats:italic>EWSR1<\/jats:italic>, <jats:italic>FUS<\/jats:italic>, <jats:italic>PBX1<\/jats:italic>, and <jats:italic>ZNF444<\/jats:italic> were investigated by fluorescence <jats:italic>in situ<\/jats:italic> hybridization. <jats:italic>EWSR1<\/jats:italic> gene rearrangement was detected in 45% of the cases. A <jats:italic>EWSR1\u2010POU5F1<\/jats:italic> fusion was identified in a pediatric soft tissue tumor by 3\u2032Rapid Amplification of cDNA Euds (RACE) and subsequently confirmed in four additional soft tissue tumors in children and young adults. An <jats:italic>EWSR1\u2010PBX1<\/jats:italic> fusion was seen in five cases, whereas <jats:italic>EWSR1\u2010ZNF444<\/jats:italic> and <jats:italic>FUS<\/jats:italic> gene rearrangement was noted in one pulmonary tumor each. In conclusion, <jats:italic>EWSR1<\/jats:italic> gene rearrangement is a common event in ME tumors arising outside salivary glands, irrespective of anatomical location. <jats:italic>EWSR1<\/jats:italic>\u2010negative tumors were more often benign, superficially located, and showed ductal differentiation, suggesting the possibility of genetically distinct groups. A subset of soft tissue ME tumors with clear cell morphology harbor an <jats:italic>EWSR1\u2010POU5F1<\/jats:italic> fusion, which can be used as a molecular diagnostic test in difficult cases. These findings do not support a pathogenetic relationship between soft tissue ME tumors and their salivary gland counterparts. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20819","type":"journal-article","created":{"date-parts":[[2010,9,3]],"date-time":"2010-09-03T19:35:19Z","timestamp":1283542519000},"page":"1114-1124","source":"Crossref","is-referenced-by-count":484,"title":["<i>EWSR1\u2010POU5F1<\/i> fusion in soft tissue myoepithelial tumors. A molecular analysis of sixty\u2010six cases, including soft tissue, bone, and visceral lesions, showing common involvement of the <i>EWSR1<\/i> gene"],"prefix":"10.1002","volume":"49","author":[{"given":"Cristina R.","family":"Antonescu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning\u2010En","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce 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