{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:59:17Z","timestamp":1761101957152},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,10,2]],"date-time":"2011-10-02T00:00:00Z","timestamp":1317513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we established and analyzed a novel human myeloid leukemia cell line, AMU\u2010AML1, from a patient with acute myeloid leukemia with multilineage dysplasia before the initiation of chemotherapy. AMU\u2010AML1 cells were positive for CD13, CD33, CD117, and HLA\u2010DR by flow cytometry analysis and showed a single chromosomal abnormality, 46, XY, t(12;22)(p13;q11.2), by G\u2010banding and spectral karyotyping. Fluorescent in situ hybridization analysis indicated that the chromosomal breakpoint in band 12p13 was in the sequence from the 5\u2032 untranslated region to intron 1 of <jats:italic>TEL<\/jats:italic> and that the chromosomal breakpoint in band 22q11 was in the 3\u2032 untranslated region of <jats:italic>MN1.<\/jats:italic> The chimeric transcript and protein of <jats:italic>MN1\u2010TEL<\/jats:italic> could not be detected by reverse\u2010transcriptase polymerase chain reaction or Western blot analysis. However, the <jats:italic>MN1<\/jats:italic> gene was amplified to three copies detected by array comparative genomic hybridization analysis, and the expression levels of the <jats:italic>MN1<\/jats:italic> transcript and protein were high in AMU\u2010AML1 cells when compared with other cell lines with t(12;22)(p13;q11\u201012). Our data showed that AMU\u2010AML1 cells contain t(12;22)(p13;q11.2) without chimeric fusion of <jats:italic>MN1<\/jats:italic> and <jats:italic>TEL<\/jats:italic>. The AMU\u2010AML1 cells gained <jats:italic>MN1<\/jats:italic> copies and had high expression levels of MN1. Thus, the AMU\u2010AML1 cell line is useful for studying the biological consequences of t(12;22)(p13;q11.2) lacking chimeric <jats:italic>MN1\u2010TEL<\/jats:italic>. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.20929","type":"journal-article","created":{"date-parts":[[2011,10,2]],"date-time":"2011-10-02T17:26:26Z","timestamp":1317576386000},"page":"42-53","source":"Crossref","is-referenced-by-count":6,"title":["Establishment of a novel human myeloid leukemia cell line, AMU\u2010AML1, carrying t(12;22)(p13;q11) without chimeric <i>MN1\u2010TEL<\/i> and with high expression of 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