{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:02:06Z","timestamp":1790809326086,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T00:00:00Z","timestamp":1322179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Burkitt lymphoma and a subset of diffuse large B\u2010cell lymphomas are characterized by chromosomal alterations affecting the <jats:italic>MYC<\/jats:italic> oncogene on 8q24. In most cases <jats:italic>MYC<\/jats:italic> is found juxtaposed to the immunoglobulin heavy chain (<jats:italic>IGH<\/jats:italic>) gene locus. Translocations to the immunoglobulin kappa (<jats:italic>IGK<\/jats:italic>) gene locus on 2p11 are observed in around 5\u201310% of cases. Little data exist on the molecular mechanisms leading to this aberration. The chromosomal breakpoints on chromosome 8 have been found dispersed over a large area 3\u2032 of <jats:italic>MYC<\/jats:italic>. In order to obtain a better understanding of this chromosomal translocation we developed a long\u2010distance inverse (LDI) PCR method for the identification of chromosomal translocations affecting the <jats:italic>IGK<\/jats:italic> locus. We investigated a number of cytogenetically mostly uncharacterized high\u2010grade lymphoma samples and identified a <jats:italic>MYC\u2013IGK<\/jats:italic> juxtaposition in seven patients and three t(2;8)\u2010positive cell lines. The chromosomal breakpoints were molecularly characterized and analyzed. The linear distance of the breakpoints on chromosome 8 to <jats:italic>MYC<\/jats:italic> ranged from some 100 bp to more than 0.5 MB. The reciprocal translocated allele could be characterized in the majority of cases. This study represents the largest series of t(2;8)\u2010positive cases analyzed so far. The LDI PCR method developed here should also be useful for the analysis of chromosomal translocations affecting the <jats:italic>IGK<\/jats:italic> locus in general. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.21915","type":"journal-article","created":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T10:00:18Z","timestamp":1322215218000},"page":"290-299","source":"Crossref","is-referenced-by-count":11,"title":["Molecular analysis of the t(2;8)\/<i>MYC\u2013IGK<\/i> translocation in high\u2010grade lymphoma\/leukemia by long\u2010distance inverse PCR"],"prefix":"10.1002","volume":"51","author":[{"given":"Hannes","family":"Kroenlein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwartz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Rieder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Molkentin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"G\u00f6kbuget","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Hoelzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Thiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Burmeister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1182\/blood.V88.3.985.bloodjournal883985","article-title":"Application 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