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Since the detection of these abnormalities through molecular\u2010genetic techniques is hampered by the overall low percentage of plasma cells present in primary bone marrow aspirates, we assessed the efficacy of these techniques in enriched plasma cell fractions from 61 multiple myeloma patients. Using interphase FISH, genomic abnormalities could be detected in 96% of the enriched samples as compared to 61% in the cultured whole bone marrow samples. We also found that microarray\u2010based genomic profiling of enriched plasma samples facilitates the detection of additional, possibly clinically relevant, genomic abnormalities. We conclude that the genomic delineation of enriched plasma cells from multiple myeloma patients results in a significantly increased detection rate of clinically relevant genomic abnormalities. In order to facilitate molecular\u2010genetic data interpretation, we recommend morphological assessment of plasma cell purity after enrichment. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.21982","type":"journal-article","created":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T15:44:30Z","timestamp":1343231070000},"page":"997-1006","source":"Crossref","is-referenced-by-count":21,"title":["High detection rate of clinically relevant genomic abnormalities in plasma cells enriched from patients with multiple myeloma"],"prefix":"10.1002","volume":"51","author":[{"given":"Marian","family":"Stevens\u2010Kroef","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Olde","family":"Weghuis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Croockewit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Derksen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen","family":"Hooijer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najat","family":"ElIdrissi\u2010Zaynoun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelique","family":"Siepman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annet","family":"Simons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ad Geurts van","family":"Kessel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20668"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.20.6136"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.28.3945"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2006.11.015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-010-0651-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402797"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.2.380.380"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-341669"},{"key":"e_1_2_6_10_1","first-page":"300","article-title":"Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells","volume":"49","author":"Fiserova A","year":"2002","journal-title":"Neoplasma"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2017"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-2876"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.174"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-203182"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00951.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2007.05.005"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.12.028"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-344069"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2009.50"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0465"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-09-379164"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20809"},{"key":"e_1_2_6_23_1","first-page":"97","article-title":"Report from the European myeloma network on interphase FISH in multiple myeloma and related disorders","author":"Ross FM","year":"2012","journal-title":"Haematologica"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(94)00284-I"},{"key":"e_1_2_6_25_1","unstructured":"ShafferLG SlovakML CampbellLJ.2009. 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