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However, differences have been observed between malignancies with identical underlying chromosomal translocations, but occurring at different age groups, suggesting some diversity in the mechanisms of formation and leukemogenesis. A multiplex long\u2010range PCR\u2010based assay was developed to allow fast and reliable amplification of patient\u2010specific<jats:italic>BCR<\/jats:italic>\u2010<jats:italic>ABL1<\/jats:italic>fusion sequences from genomic DNA. The localization of breakpoints was analyzed with respect to distribution within the breakpoint cluster regions, sequence features, and association to repetitive elements or motifs associated with DNA recombination. The genomic fusion sites of 59 pediatric CML patients showed a bimodal breakpoint distribution in<jats:italic>BCR<\/jats:italic>that was different from the distribution in adult CML cases, but with similarities to<jats:italic>BCR<\/jats:italic>\u2010<jats:italic>ABL1\u2010<\/jats:italic>positive, acute lymphoblastic leukemia in adults.<jats:italic>BCR<\/jats:italic>breakpoints were found more frequently positioned within, or close to,<jats:italic>Alu<\/jats:italic>repeats than would be expected based on their overall sequence proportion. Technical aspects of the highly sensitive DNA\u2010based quantification of residual CML cells by specific fusion sequence during tyrosine kinase inhibitor therapy are exemplified in a subcohort of pediatric CML patients. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.21989","type":"journal-article","created":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T10:49:09Z","timestamp":1344509349000},"page":"1045-1053","source":"Crossref","is-referenced-by-count":60,"title":["Genomic<i>BCR<\/i>\u2010<i>ABL1<\/i>breakpoints in pediatric chronic myeloid leukemia"],"prefix":"10.1002","volume":"51","author":[{"given":"Manuela","family":"Krumbholz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Karl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine 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