{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:24:39Z","timestamp":1783016679145,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T00:00:00Z","timestamp":1344470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously shown that the <jats:italic>E2F3<\/jats:italic> oncogene is up\u2010regulated as part of a \u201cpreneoplastic expression profile\u201d in fallopian tube epithelium (FTE) of women with <jats:italic>BRCA1<\/jats:italic> mutations. We studied <jats:italic>E2F3<\/jats:italic> expression in FTE and carcinomas of women with <jats:italic>BRCA1<\/jats:italic> or <jats:italic>BRCA2<\/jats:italic> mutations or wildtype for both genes. Significantly more foci of TP53 positive cells in histologically normal FTE from women with <jats:italic>BRCA1<\/jats:italic> mutations but not in wildtype or <jats:italic>BRCA2<\/jats:italic> mutated individuals had E2F3 protein overexpression relative to adjacent normal FTE, which occurred in the context of focally increased proliferation, potentially explaining the increased neoplastic potential of tubal TP53 foci in women with <jats:italic>BRCA1<\/jats:italic> mutations. To assess mechanisms of <jats:italic>E2F3<\/jats:italic> deregulation in ovarian or tubal carcinogenesis, we studied <jats:italic>E2F3<\/jats:italic> and its two isoforms E2F3a and E2F3b in wild\u2010type ovarian carcinomas and ovarian carcinomas associated with germline <jats:italic>BRCA1<\/jats:italic> and <jats:italic>BRCA2<\/jats:italic> mutations. The expression of <jats:italic>E2F3b<\/jats:italic>, but not <jats:italic>E2F3a<\/jats:italic>, was correlated with the expression of <jats:italic>BRCA1<\/jats:italic> in all three genetic groups. In primary cultures of FTE from women with <jats:italic>BRCA1<\/jats:italic> mutation or wildtype for <jats:italic>BRCA1<\/jats:italic> and <jats:italic>BRCA2<\/jats:italic>, siRNA\u2010induced <jats:italic>BRCA1<\/jats:italic> deficiency led to increased <jats:italic>E2F3b<\/jats:italic> but not <jats:italic>E2F3a<\/jats:italic> expression. Our results suggest that <jats:italic>E2F3b<\/jats:italic> and <jats:italic>BRCA1<\/jats:italic> are functionally connected, and <jats:italic>BRCA1<\/jats:italic> haploinsufficiency in normal FTE may lead to up\u2010regulation of <jats:italic>E2F3b<\/jats:italic> and increased proliferation before the development of intraepithelial neoplasia. These data support that <jats:italic>E2F3b<\/jats:italic> up\u2010regulation is an important preneoplastic event in FTE from <jats:italic>BRCA1<\/jats:italic> mutation carriers. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.21990","type":"journal-article","created":{"date-parts":[[2012,8,9]],"date-time":"2012-08-09T10:49:17Z","timestamp":1344509357000},"page":"1054-1062","source":"Crossref","is-referenced-by-count":4,"title":["<i>E2F3b<\/i> over\u2010expression in ovarian carcinomas and in <i>BRCA1<\/i> Haploinsufficient fallopian tube epithelium"],"prefix":"10.1002","volume":"51","author":[{"given":"Na Lu","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piri","family":"Welcsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua Z.","family":"Press","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy J.","family":"Agnew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rochelle","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth 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