{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:51:04Z","timestamp":1787755864606,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T00:00:00Z","timestamp":1367280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>Mesothelioma is a rare but very aggressive tumor derived from mesothelial cells. A number of often complex but nonrandom cytogenetic abnormalities have been found in these tumors, resulting in loss of chromosome bands 14q32 and 22q12 in more than 35% of the cases. In this study, we used RNA sequencing to search for fusion transcripts in a mesothelioma carrying a t(14;22)(q32;q12) as the sole chromosomal aberration and found an<jats:italic>EWSR1\u2013YY1<\/jats:italic>and its reciprocal<jats:italic>YY1\u2013EWSR1<\/jats:italic>fusion transcript. Screening 15 additional cases of mesothelioma from which we had RNA but no cytogenetic information, we identified one more tumor carrying an<jats:italic>EWSR1\u2013YY1<\/jats:italic>fusion gene but not the reciprocal<jats:italic>YY1\u2013EWSR1<\/jats:italic>transcript. RT\u2010polymerase chain reaction and sequencing showed that in both cases exon 8 of<jats:italic>EWSR1<\/jats:italic>(nucleotide 1,139, accession number NM_013986 version 3, former exon 7 in sequence with accession number X66899) was fused to exon 2 of<jats:italic>YY1<\/jats:italic>(nucleotide 1,160, accession number NM_003403 version 3). The<jats:italic>EWSR1<\/jats:italic>breakpoint in exon 8 in the<jats:italic>EWSR1\u2013YY1<\/jats:italic>chimeric transcript is similar to what is found in other fusions involving<jats:italic>EWSR1<\/jats:italic>such as<jats:italic>EWSR1\u2013FLI1<\/jats:italic>,<jats:italic>EWSR1\u2013DDIT3<\/jats:italic>, and<jats:italic>EWSR1\u2013ATF1<\/jats:italic>. The EWSR1\u2013YY1\u2010encoded protein is an abnormal transcription factor with the transactivation domain of EWSR1 and the DNA\u2010binding domain of YY1. This is the first study to detect a specific fusion gene in mesothelioma (the reason how frequent the<jats:italic>EWSR1\u2013YY1<\/jats:italic>fusion is remains uncertain) and also the first time that direct involvement of YY1 in oncogenesis has been demonstrated. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22068","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T05:28:33Z","timestamp":1367299713000},"page":"733-740","source":"Crossref","is-referenced-by-count":61,"title":["RNA sequencing identifies fusion of the<i>EWSR1<\/i>and<i>YY1<\/i>genes in mesothelioma with t(14;22)(q32;q12)"],"prefix":"10.1002","volume":"52","author":[{"given":"Ioannis","family":"Panagopoulos","sequence":"first","affiliation":[{"name":"Section for Cancer Cytogenetics Institute for Medical Informatics, The Norwegian Radium Hospital, Oslo University Hospital Oslo Norway"},{"name":"Centre for Cancer Biomedicine Faculty of Medicine, University of Oslo 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