{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T15:38:28Z","timestamp":1776872308845,"version":"3.51.2"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Uterine leiomyomas (UL) are the most frequent symptomatic human tumors. Nevertheless, their molecular pathogenesis is not yet fully understood. To learn more about the biology of these common neoplasms and their response to treatment, cell cultures derived from UL are a frequently used model system, but until recently appropriate genetic markers confirming their origin from the tumor cell population were lacking for most UL, i.e., those not displaying karyotypic abnormalities. The identification of <jats:italic>MED12<\/jats:italic> mutations in the majority of UL makes it possible to trace the tumor cell population during in vitro passaging in the absence of cytogenetic abnormalities. The present study is addressing the in vitro survival of cells carrying <jats:italic>MED12<\/jats:italic> mutations and its association with karyotypic alterations. The results challenge numerous in vitro studies into the biology and behavior of leiomyomas. Cells of one genetic subtype of UL, i.e., those with rearrangements of the high mobility AT\u2010hook 2 protein gene (<jats:italic>HMGA2<\/jats:italic>), seem to be able to proliferate in vitro for many passages whereas tumor cells from the much more frequent <jats:italic>MED12<\/jats:italic>\u2010mutated lesions barely survive even the first passages. Apparently, for the most frequent type of human UL no good in vitro model seems to exist because cells do not survive culturing. On the other hand, this inability may point to an Achilles' heel of this type of UL. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22142","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T05:41:13Z","timestamp":1390282873000},"page":"317-323","source":"Crossref","is-referenced-by-count":29,"title":["Cell cultures in uterine leiomyomas: Rapid disappearance of cells carrying <i>MED12<\/i> mutations"],"prefix":"10.1002","volume":"53","author":[{"given":"Dominique","family":"Nadine Markowski","sequence":"first","affiliation":[{"name":"Center for Human Genetics, University of Bremen Leobener Str. ZHG 28359 Bremen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahboobeh","family":"Tadayyon","sequence":"additional","affiliation":[{"name":"Center for Human Genetics, University of Bremen Leobener Str. ZHG 28359 Bremen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Bartnitzke","sequence":"additional","affiliation":[{"name":"Center for Human Genetics, University of Bremen Leobener Str. ZHG 28359 Bremen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gazanfer","family":"Belge","sequence":"additional","affiliation":[{"name":"Center for Human Genetics, University of Bremen Leobener Str. ZHG 28359 Bremen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhard","family":"Maria Helmke","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rn","family":"Bullerdiek","sequence":"additional","affiliation":[{"name":"Center for Human Genetics, University of Bremen Leobener Str. ZHG 28359 Bremen Germany"},{"name":"Institute for Medical Genetics, University of Rostock, University Medicine Ernst\u2010Heydemann\u2010Str. 8 18057 Rostock Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.02.136"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000017499.51216.3E"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2010.05.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719109344773"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719109348924"},{"key":"e_1_2_6_7_1","first-page":"36","article-title":"Growth potential of human uterine leiomyomas: Some in vitro observations and their implications","volume":"66","author":"Cramer SF","year":"1985","journal-title":"Obstet Gynecol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02915092"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20627"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719107314063"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208930"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2010.06.010"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20871"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27424"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000096"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.04.044"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870110102"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1302736"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det275"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036935"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313650110"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2004.08.025"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0895-436"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.12.011"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2006.05.003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1251483"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal018"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.22142","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.22142","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T08:59:45Z","timestamp":1696582785000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.22142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,21]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1002\/gcc.22142"],"URL":"https:\/\/doi.org\/10.1002\/gcc.22142","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,21]]}}}