{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:43:28Z","timestamp":1791085408053,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:p>Eosinophilia\u2010associated myeloid neoplasms with rearrangement of chromosome bands 5q31\u201033 are frequently associated with <jats:italic>PDGFRB<\/jats:italic> fusion genes, which are exquisitely sensitive to treatment with imatinib. In search for novel fusion partners of <jats:italic>PDGFRB<\/jats:italic>, we analyzed three cases with translocation t(5;20)(q33;p11), t(5;14)(q33;q32), and t(5;17;14)(q33;q11;q32) by 5\u2032\u2010rapid amplification of cDNA ends polymerase chain reaction (5\u2032\u2010RACE\u2010PCR) and DNA\u2010based long\u2010distance inverse PCR (LDI\u2010PCR) with primers derived from <jats:italic>PDGFRB<\/jats:italic>. LDI\u2010PCR revealed a fusion between <jats:italic>CCDC88C<\/jats:italic> exon 25 and <jats:italic>PDGFRB<\/jats:italic> exon 11 in the case with t(5;17;14)(q33;q11;q32) while 5\u2032\u2010RACE\u2010PCR identified fusions between <jats:italic>CCDC88C<\/jats:italic> exon 10 and <jats:italic>PDGFRB<\/jats:italic> exon 12 and between <jats:italic>DTD1<\/jats:italic> exon 4 and <jats:italic>PDGFRB<\/jats:italic> exon 12 in the cases with t(5;14)(q33;q32) and t(5;20)(q33;p11), respectively. The PDGFRB tyrosine\u2010kinase domain is predicted to be retained in all three fusion proteins. The partner proteins contained coiled\u2010coil domains or other domains, which putatively lead to constitutive activation of the PDGFRB fusion protein. In vitro functional analyses confirmed transforming activity and imatinib\u2010sensitivity of the fusion proteins. All three patients achieved rapid and durable complete hematologic remissions on imatinib. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22153","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:34:10Z","timestamp":1392208450000},"page":"411-421","source":"Crossref","is-referenced-by-count":27,"title":["Identification and functional characterization of imatinib\u2010sensitive <i>DTD1\u2010PDGFRB<\/i> and <i>CCDC88C\u2010PDGFRB<\/i> fusion genes in eosinophilia\u2010associated myeloid\/lymphoid neoplasms"],"prefix":"10.1002","volume":"53","author":[{"given":"Darko","family":"Gosenca","sequence":"first","affiliation":[{"name":"III. Medizinische Klinik Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Kellert","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Dermatologie, Venerologie und Allergologie Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia","family":"Metzgeroth","sequence":"additional","affiliation":[{"name":"III. Medizinische Klinik Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Haferlach","sequence":"additional","affiliation":[{"name":"MLL M\u00fcnchner Leuk\u00e4mie Labor GmbH M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Fabarius","sequence":"additional","affiliation":[{"name":"III. 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Medizinische Klinik Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kneba","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Innere Medizin II Universit\u00e4tsklinikum Schleswig\u2010Holstein Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Scheid","sequence":"additional","affiliation":[{"name":"Klinik I f\u00fcr Innere Medizin Uniklinik K\u00f6ln Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"T\u00f6pelt","sequence":"additional","affiliation":[{"name":"Klinik I f\u00fcr Innere Medizin Uniklinik K\u00f6ln Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Erben","sequence":"additional","affiliation":[{"name":"III. Medizinische Klinik Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Haferlach","sequence":"additional","affiliation":[{"name":"MLL M\u00fcnchner Leuk\u00e4mie Labor GmbH M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas C. P.","family":"Cross","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Southampton Southampton UK"},{"name":"Wessex Regional Genetics Laboratory Salisbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf\u2010Karsten","family":"Hofmann","sequence":"additional","affiliation":[{"name":"III. Medizinische Klinik Universit\u00e4tsmedizin Mannheim Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Seifarth","sequence":"additional","affiliation":[{"name":"III. 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