{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:04:09Z","timestamp":1762542249294},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T00:00:00Z","timestamp":1393891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Burkitt lymphoma cell lines (BL\u2010CL) are used extensively as in vitro models in genetic studies; however, cytogenetic information is not always available or updated. We provide a comprehensive cytogenetic resource of 44 BL\u2010CL, assessed by G\u2010banding, multicolor\u2010FISH, and FISH with 1q, 3p, 7q, and 13q region\u2010specific probes, including the first cytogenetic characterization of 22 BL\u2010CL and the revision of further 22 commonly used BL\u2010CL. Based on these data, we determined a consensus karyotype, evaluated in detail the secondary chromosomal changes (SCC), and the karyotypic stability of these cell lines. An individual karyotype was identified in all investigated BL\u2010CL, confirming their unique origin. Most of the BL\u2010CL remained cytogenetically relative stable after years of intensive cultivation. The most frequent structural SCC were dup(1q), del(13q) and the most frequent numerical SCC were +7, +13. Common breakpoints were located on 1q12, 7q11, and 13q31. The most common gains were in 1q and 7q and the most common losses were in 11q and 13q. Interestingly, the frequency of 1q gains and 13q losses was significantly higher in the EBV\u2010negative than in the EBV\u2010positive BL\u2010CL. Furthermore, by reviewing karyotypes of 221 primary BL listed in the Mitelman database, we observed similarities between BL\u2010CL and primary BL regarding the frequency of numerical and structural SCC and breakpoint distribution. In BL\u2010CL and in primary BL two SCC, dup(1q), and +12, always occurred mutually exclusive of each other. These findings validate BL\u2010CL as appropriate model for in vitro studies on the significance of SCC in the pathogenesis of BL. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22161","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T07:48:44Z","timestamp":1393919324000},"page":"497-515","source":"Crossref","is-referenced-by-count":20,"title":["Comprehensive cytogenetic and molecular cytogenetic analysis of 44 Burkitt lymphoma cell lines: Secondary chromosomal changes characterization, karyotypic evolution, and comparison with primary samples"],"prefix":"10.1002","volume":"53","author":[{"given":"Eva","family":"Maria Murga Penas","sequence":"first","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"},{"name":"Institute of Human Genetics, Christian\u2010Albrechts\u2010University of Kiel &amp; University Hospital Schleswig\u2010Holstein Campus Kiel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Schilling","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Behrmann","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Klokow","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eik","family":"Vettorazzi","sequence":"additional","affiliation":[{"name":"Department of Medical Biometry and Epidemiology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Bokemeyer","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Dierlamm","sequence":"additional","affiliation":[{"name":"Department of Oncology and Hematology BMT with Section Pneumology, Hubertus Wald Cancer Center, University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2852"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-297879"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1596"},{"key":"e_1_2_6_5_1","first-page":"143","article-title":"Is there a functional equivalence between abnormalities of the long arm of chromosome 1 and the presence of Epstein\u2010Barr virus in continuous lines of Burkitt's lymphoma?","volume":"298","author":"Berger R","year":"1984","journal-title":"C R Acad Sci III"},{"key":"e_1_2_6_6_1","first-page":"65","article-title":"Cytogenetics of Burkitt's lymphoma\u2010leukaemia: A review","volume":"60","author":"Berger R","year":"1985","journal-title":"IARC Sci Publ"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.281"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.24.7824"},{"key":"e_1_2_6_9_1","first-page":"2431","article-title":"Lymphoma\/Leukemia Molecular Profiling Project. 2006. 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