{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:51:30Z","timestamp":1789206690780,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T00:00:00Z","timestamp":1410998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>The genetics of myoepithelial tumors (ME) of soft tissue and bone have recently been investigated, with <jats:italic>EWSR1<\/jats:italic>\u2010related gene fusions being seen in approximately half of the tumors. The fusion partners of <jats:italic>EWSR1<\/jats:italic> so far described include <jats:italic>POU5F1<\/jats:italic>, <jats:italic>PBX1<\/jats:italic>, <jats:italic>ZNF444<\/jats:italic> and, in a rare case, <jats:italic>ATF1<\/jats:italic>. We investigated by RNA sequencing an index case of <jats:italic>EWSR1<\/jats:italic>\u2010rearranged ME of the tibia, lacking a known fusion partner, and identified a novel <jats:italic>EWSR1\u2010PBX3<\/jats:italic> fusion. The fusion was further validated by reverse transcriptase polymerase chain reaction and fluorescence in situ hybridization (FISH). To evaluate if this is a recurrent event, an additional cohort of 22 <jats:italic>EWSR1<\/jats:italic>\u2010rearranged ME cases lacking a fusion partner were screened by FISH for abnormalities in <jats:italic>PBX3<\/jats:italic> gene. Thus, two additional cases were identified showing an <jats:italic>EWSR1\u2010PBX3<\/jats:italic> gene fusion. One of them was also intraosseous involving the ankle, while the other occurred in the soft tissue of the index finger. The morphology of the <jats:italic>EWSR1\u2010PBX3<\/jats:italic> fusion positive cases showed similar findings, with nests or sheets of epithelioid to spindle cells in a partially myxoid to collagenous matrix. All three cases showed expression of S100 and EMA by immunohistochemistry. In summary, we report a novel <jats:italic>EWSR1\u2010PBX3<\/jats:italic> gene fusion in a small subset of ME, thereby expanding the spectrum of <jats:italic>EWSR1<\/jats:italic>\u2010related gene fusions seen in these tumors. This gene fusion seems to occur preferentially in skeletal ME, with two of the three study cases occurring in intraosseous locations. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22216","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T06:36:17Z","timestamp":1411022177000},"page":"63-71","source":"Crossref","is-referenced-by-count":98,"title":["<i>EWSR1\u2010PBX3<\/i>: A novel gene fusion in myoepithelial tumors"],"prefix":"10.1002","volume":"54","author":[{"given":"Narasimhan P.","family":"Agaram","sequence":"first","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao\u2010Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Shao","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Panicek","sequence":"additional","affiliation":[{"name":"Department of Radiology Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Healey","sequence":"additional","affiliation":[{"name":"Department of Surgery Orthopedic Service, Memorial Sloan Kettering Cancer Center New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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