{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:41:06Z","timestamp":1788871266986,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondo de Investigaci\u00c3\u00b3n Sanitaria, Ministerio de Ciencia e Innovaci\u00c3\u00b3n, Spain","award":["PI13\/00055"],"award-info":[{"award-number":["PI13\/00055"]}]},{"name":"Fondo de Investigaci\u00c3\u00b3n Sanitaria, Ministerio de Ciencia e Innovaci\u00c3\u00b3n, Spain","award":["PI13\/00800"],"award-info":[{"award-number":["PI13\/00800"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>\n                    Schwannomas are tumors that develop from Schwann cells in the peripheral nerves and commonly arise from the vestibular nerve. Vestibular schwannomas can present unilaterally and sporadically or bilaterally when the tumor is associated with neurofibromatosis Type 2 (NF2) syndrome. The molecular hallmark of the disease is biallelic inactivation of the\n                    <jats:italic>NF2<\/jats:italic>\n                    gene. The epigenetic signature of schwannomas remains poorly understood and is mostly limited to DNA methylation of the\n                    <jats:italic>NF2<\/jats:italic>\n                    gene, whose altered expression due to epigenetic factors in this tumor is controversial. In this study, we tested the genomewide DNA methylation pattern of schwannomas to shed light on this epigenetic alteration in these particular tumors. The methodology used includes Infinium Human Methylation 450K BeadChip microarrays in a series of 36 vestibular schwannomas, 4 nonvestibular schwannomas, and 5 healthy nerves. Our results show a trend toward hypomethylation in schwannomas. Furthermore, homeobox (HOX) genes, located at four clusters in the genome, displayed hypomethylation in several CpG sites in the vestibular schwannomas but not in the nonvestibular schwannomas. Several microRNA (miRNA) and protein\u2010coding genes were also found to be hypomethylated at promoter regions and were confirmed as upregulated by expression analysis; including miRNA\u201021, Met Proto\u2010Oncogene (\n                    <jats:italic>MET<\/jats:italic>\n                    ), and\n                    <jats:italic>PMEPA1<\/jats:italic>\n                    . We also detected methylation patterns that might be involved in alternative transcripts of several genes such as\n                    <jats:italic>NRXN1<\/jats:italic>\n                    or\n                    <jats:italic>MBP<\/jats:italic>\n                    , which would increase the complexity of the methylation and expression patterns. Overall, our results show specific epigenetic signatures in several coding genes and miRNAs that could potentially be used as therapeutic targets. \u00a9 2014 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/gcc.22232","type":"journal-article","created":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T02:37:36Z","timestamp":1419302256000},"page":"197-209","source":"Crossref","is-referenced-by-count":20,"title":["Genome\u2010wide methylation analysis in vestibular schwannomas shows putative mechanisms of gene expression modulation and global hypomethylation at the HOX gene cluster"],"prefix":"10.1002","volume":"54","author":[{"given":"Miguel","family":"Torres\u2010Mart\u00edn","sequence":"first","affiliation":[{"name":"Molecular Neuro\u2010oncogenetics Laboratory Research Unit, Hospital Universitario La Paz IdiPAZ Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Lassaletta","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Hospital Universitario La Paz IdiPAZ Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M","family":"de Campos","sequence":"additional","affiliation":[{"name":"Neurosurgery Department Fundacion Jimenez Diaz  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Isla","sequence":"additional","affiliation":[{"name":"Neurosurgery Department Hospital Universitario La Paz IdiPAZ Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanny R.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Biology Laboratory Federal University of Piau  Parnaiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rommel R.","family":"Burbano","sequence":"additional","affiliation":[{"name":"Human Cytogenetics Laboratory Institute of Biological Sciences, Federal University of Para  Belem Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara","family":"Melendez","sequence":"additional","affiliation":[{"name":"Molecular Pathology Research Unit Virgen de la Salud Hospital  Toledo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier S.","family":"Castresana","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics University of Navarra School of Sciences  Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Rey","sequence":"additional","affiliation":[{"name":"Molecular Neuro\u2010oncogenetics Laboratory Research Unit, Hospital Universitario La Paz IdiPAZ Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1227\/NEU.0b013e3181ec7b71"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc907"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4025"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210760"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-0622"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870060403"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2006.02.022"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22767"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1181-y"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nou020"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24438"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141121727"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.23470"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25941"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/77083"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004160"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-012-1342-8"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2012.05.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/mao.0b013e31815d4429"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.471"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn224"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-587"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200011093431901"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgp250"},{"key":"e_1_2_6_26_1","first-page":"5601","article-title":"CpG 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