{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:00:59Z","timestamp":1780491659563,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T00:00:00Z","timestamp":1419552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001862","name":"Swedish Research Council Formas","doi-asserted-by":"publisher","award":["K2009-66X;"],"award-info":[{"award-number":["K2009-66X;"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000183","name":"Army Research Office","doi-asserted-by":"publisher","award":["W81XWH-11-1- 0747"],"award-info":[{"award-number":["W81XWH-11-1- 0747"]}],"id":[{"id":"10.13039\/100000183","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001006","name":"Breast Cancer Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001006","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Helse S\u00c3\u00b8r-\u00c3\u02dcst"},{"name":"K.G. 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We developed a quantitative multigene fluorescence <jats:italic>in situ<\/jats:italic> hybridization (QM FISH) method to detect multiple genomic regions in single cells in complex tissues. As a \u201cproof of principle\u201d we applied the method to breast cancer samples to identify partners in whole arm (WA) translocations. WA gain of chromosome arm 1q and loss of chromosome arm 16q are among the most frequent genomic events in breast cancer. By designing five specific FISH probes based on breakpoint information from comparative genomic hybridization array (aCGH) profiles, we visualized chromosomal translocations in clinical samples at the single cell level. By analyzing aCGH data from 295 patients with breast carcinoma with known molecular subtype, we found concurrent WA gain of 1q and loss of 16q to be more frequent in luminal A tumors compared to other molecular subtypes. QM FISH applied to a subset of samples (<jats:italic>n<\/jats:italic>\u2009=\u200926) identified a derivative chromosome der(1;16)(q10;p10), a result of a centromere\u2010close translocation between chromosome arms 1q and 16p. In addition, we observed that the distribution of cells with the translocation varied from sample to sample, some had a homogenous cell population while others displayed intratumor heterogeneity with cell\u2010to\u2010cell variation. Finally, for one tumor with both preinvasive and invasive components, the fraction of cells with translocation was lower and more heterogeneous in the preinvasive tumor cells compared to the cells in the invasive component. \u00a9 2014 The Authors Genes, Chromosomes &amp; Cancer Published by Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22237","type":"journal-article","created":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T15:11:18Z","timestamp":1419606678000},"page":"235-248","source":"Crossref","is-referenced-by-count":15,"title":["Quantitative multigene FISH on breast carcinomas identifies der(1;16)(q10;p10) as an early event in luminal A tumors"],"prefix":"10.1002","volume":"54","author":[{"given":"Inga H.","family":"Rye","sequence":"first","affiliation":[{"name":"Department of Genetics Institute for Cancer Research Oslo University Hospital Radiumhospitalet 0424 Oslo 0310 Norway"},{"name":"The K.G. 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Jebsen Center for Breast Cancer Research Institute for Clinical Medicine, Faculty of Medicine, University of Oslo  Oslo Norway"},{"name":"Department of Oncology Oslo University Hospital Radiumhospitalet 0424 Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Wigler","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory Cold Spring Harbor  New York 11724 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Hicks","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory Cold Spring Harbor  New York 11724 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Lise","family":"B\u00f8rresen\u2010Dale","sequence":"additional","affiliation":[{"name":"Department of Genetics Institute for Cancer Research Oslo University Hospital Radiumhospitalet 0424 Oslo 0310 Norway"},{"name":"The K.G. Jebsen Center for Breast Cancer Research Institute for Clinical Medicine, Faculty of Medicine, University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Zetterberg","sequence":"additional","affiliation":[{"name":"Department of Oncology\u2013Pathology Karolinska Institutet, Cancer Center Karolinska SE\u2010171 76 Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hege G.","family":"Russnes","sequence":"additional","affiliation":[{"name":"Department of Genetics Institute for Cancer Research Oslo University Hospital Radiumhospitalet 0424 Oslo 0310 Norway"},{"name":"The K.G. Jebsen Center for Breast Cancer Research Institute for Clinical Medicine, Faculty of Medicine, University of Oslo  Oslo Norway"},{"name":"Department of Pathology Oslo University Hospital Radiumhospitalet 0424 Oslo 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