{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:22:06Z","timestamp":1740104526690,"version":"3.37.3"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,23]],"date-time":"2015-02-23T00:00:00Z","timestamp":1424649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Competitive Research Funding of Tampere University Hospital"},{"name":"Finnish Cancer Society"},{"DOI":"10.13039\/100008723","name":"Finnish Medical Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008723","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tampere Medical Society"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>Mutations in the <jats:italic>BRCA1<\/jats:italic> and <jats:italic>BRCA2<\/jats:italic> genes confer an increased lifetime risk for breast and ovarian cancer. Ovarian cancer risk can be decreased by risk\u2010reducing salpingo\u2010oophorectomy (RRSO). Studies on RRSO material have altered the paradigm of serous ovarian cancer pathogenesis. The purpose of this study was to identify candidate genes possibly involved in the pathogenesis of serous ovarian cancer by carrying out a microarray analysis of differentially expressed genes in <jats:italic>BRCA1\/2<\/jats:italic>\u2010 mutation positive ovarian and fallopian tube epithelium derived from RRSO surgery. Freshly frozen ovarian and fallopian tube samples from nine <jats:italic>BRCA1\/2<\/jats:italic> mutation carriers scheduled for RRSO were prospectively collected together with five mutation\u2010negative control patients undergoing salpingo\u2010oophorectomy for benign indications. Microarray analysis of genome\u2010wide gene expression was performed on ovarian and fallopian tube samples from the <jats:italic>BRCA1\/2<\/jats:italic> and control patients. The validation of microarray data was performed by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) in selected cases of RRSO samples and also in high grade serous carcinoma samples collected from patients with a <jats:italic>BRCA<\/jats:italic> phenotype. From 22,733 genes, 454 transcripts were identified that were differentially expressed in <jats:italic>BRCA1\/2<\/jats:italic> mutation carriers when compared with controls, pooling all ovarian and fallopian tube samples together. Of these, 299 genes were statistically significantly downregulated and 155 genes upregulated. Differentially expressed genes in <jats:italic>BRCA1\/2<\/jats:italic> samples reported here might be involved in serous ovarian carcinogenesis and provide interesting targets for further studies. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22241","type":"journal-article","created":{"date-parts":[[2015,2,23]],"date-time":"2015-02-23T11:40:15Z","timestamp":1424691615000},"page":"276-287","source":"Crossref","is-referenced-by-count":4,"title":["Microarray analysis of differentially expressed genes in ovarian and fallopian tube epithelium from risk\u2010reducing salpingo\u2010oophorectomies"],"prefix":"10.1002","volume":"54","author":[{"given":"Kristina","family":"Veskim\u00e4e","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Tampere University Hospital Tampere Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Synn\u00f6ve","family":"Staff","sequence":"additional","affiliation":[{"name":"Department of 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