{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:16:23Z","timestamp":1788434183844,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T00:00:00Z","timestamp":1437955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Secci\u00f3n Catalana de Dermatolog\u00eda de \u201cl\u2019Acad\u00e8mia Espa\u00f1ola de Dermatolog\u00eda y Venereolog\u00eda\u201d i la Societat Catalana de Dermatologia de l\u2019Acad\u00e8mia de Ci\u00e8ncies M\u00e8diques i de la Salut de Catalunya"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>There is a lack of studies on somatic gene mutations and cell signaling driving penile carcinogenesis. Our objective was to analyze somatic mutations in genes downstream of EGFR in penile squamous cell carcinomas, especially the mTOR and RAS\/MAPK pathways. We retrospectively analyzed somatic mutations in 10 in situ and 65 invasive penile squamous cell carcinomas by using Sequenom's Mass Spectrometry iPlex Technology and Oncocarta v1.0 Panel. The DNA was extracted from FFPE blocks and we identified somatic missense mutations in three in situ tumors and in 19 invasive tumors, mostly in <jats:italic>PIK3CA<\/jats:italic>, <jats:italic>KRAS<\/jats:italic>, <jats:italic>HRAS, NRAS<\/jats:italic>, and <jats:italic>PDGFA<\/jats:italic> genes. Somatic mutations in the <jats:italic>PIK3CA<\/jats:italic> gene or <jats:italic>RAS<\/jats:italic> family genes were neither associated with tumor grade, stage or outcome, and were equally often identified in hrHPV positive and in hrHPV negative tumors that showed no p53 expression. Mutations in <jats:italic>PIK3CA, KRAS<\/jats:italic>, and <jats:italic>HRAS<\/jats:italic> are frequent in penile squamous cell carcinoma and likely play a role in the development of p53\u2010negative tumors. Although the presence of these mutations does not seem to correlate with tumoral behavior or outcome, they could be biomarkers of treatment failure with anti\u2010EGFR mAb in patients with penile squamous cell carcinoma. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22274","type":"journal-article","created":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T08:23:00Z","timestamp":1437985380000},"page":"629-637","source":"Crossref","is-referenced-by-count":20,"title":["Identification of somatic gene mutations in penile squamous cell carcinoma"],"prefix":"10.1002","volume":"54","author":[{"given":"Carla","family":"Ferr\u00e1ndiz\u2010Pulido","sequence":"first","affiliation":[{"name":"Dermatology Department Hospital Universitari Vall D'hebron Barcelona Spain"},{"name":"Faculty of Medicine Universitat Aut\u00f2noma De Barcelona Barcelona 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