{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:14:16Z","timestamp":1764782056709},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T00:00:00Z","timestamp":1441843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>In myelodysplastic syndromes (MDS), deletion of the short arm of chromosome 12 (del(12p)) is usually a small abnormality, rarely detected as a single aberration by chromosome banding analysis (CBA) of bone marrow metaphases. Del(12p) has been described in 0.6 to 5% of MDS patients at initial diagnosis and is associated with a good to intermediate prognosis as a sole anomaly according to current scoring systems. Here, we present the results of a systematic del(12p) testing in a German prospective diagnostic study (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">clinicaltrials.gov<\/jats:ext-link>: NCT01355913) on 367 MDS patients in whom CD34+ peripheral blood cells were analysed for the presence of del(12p) by sequential fluorescence in situ hybridization (FISH) analyses. A cohort of 2,902 previously published MDS patients diagnosed by CBA served as control. We demonstrate that, using a sensitive FISH technique, 12p deletion occurs significantly more frequently in MDS than previously described (7.6% by CD34+ PB\u2010FISH vs. 1.6% by CBA, <jats:italic>P &lt;<\/jats:italic> 0.001) and is often associated with other aberrations (93% by CD34+ PB\u2010FISH vs. 60% by CBA). Additionally, the detection rate can be increased by repeated analyses in a patient over time which is important for the patient\u00b4s prognosis to distinguish a sole anomaly from double or complex aberrations. To our knowledge, this is the first study to screen for 12p deletions with a suitable probe for <jats:italic>ETV6\/TEL<\/jats:italic> in 12p13. Our data suggest that the supplement of a probe for the detection of a 12p deletion to common FISH probe panels helps to avoid missing a del(12p), especially as part of more complex aberrations. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22292","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T23:10:47Z","timestamp":1441926647000},"page":"809-817","source":"Crossref","is-referenced-by-count":9,"title":["Frequency of del(12p) is commonly underestimated in myelodysplastic syndromes: Results from a <scp>G<\/scp>erman diagnostic study in comparison with an international control group"],"prefix":"10.1002","volume":"54","author":[{"given":"Friederike","family":"Braulke","sequence":"first","affiliation":[{"name":"Department of Hematology and Medical Oncology University Medicine of Goettingen  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharina","family":"M\u00fcller\u2010Thomas","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Technical University of Munich  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"G\u00f6tze","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Technical University of Munich  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Platzbecker","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University of Dresden  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Germing","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University of Duesseldorf  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf\u2010Karsten","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University Hospital of Mannheim  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristoteles A. N.","family":"Giagounidis","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Marienhospital Duesseldorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"L\u00fcbbert","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University of Freiburg Medical Center Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter L.","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Department of Hematology Stanford University Cancer Center Stanford CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Bennett","sequence":"additional","affiliation":[{"name":"University of Rochester Medical Center Rochester NY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesc","family":"Sol\u00e9","sequence":"additional","affiliation":[{"name":"Institut De Recerca Contra La Leukemia Josep Carreras Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn L.","family":"Slovak","sequence":"additional","affiliation":[{"name":"Sonora Quest Laboratories Phoenix AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuma","family":"Ohyashiki","sequence":"additional","affiliation":[{"name":"Tokyo Medical University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle M.","family":"Le Beau","sequence":"additional","affiliation":[{"name":"Section of Hematology and Oncology University of Chicago Chicago IL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"T\u00fcchler","sequence":"additional","affiliation":[{"name":"Hanusch Hospital Boltzmann Institute for Leukemia Research Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Pfeilst\u00f6cker","sequence":"additional","affiliation":[{"name":"Third Medical Department for Hematology and Oncology and L. Boltzmann Cluster Oncology Hanusch Hospital Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Hildebrandt","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of D\u00fcsseldorf D\u00fcsseldorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Aul","sequence":"additional","affiliation":[{"name":"Department of Hematology Oncology and Clinical Immunology, St. Johannes Hospital Duisburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Stauder","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Innsbruck Medical University Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Valent","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Hematology and Hemostaseology and Ludwig Boltzmann Cluster Oncology 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