{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T13:48:43Z","timestamp":1764510523997},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T00:00:00Z","timestamp":1441843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>To clarify whether karyotype aberrations (KA) involving regions not covered by the standard fluorescence in situ hybridization (FISH) panel have independent prognostic relevance, we evaluated KA by conventional cytogenetics in a learning cohort (LC; <jats:italic>n<\/jats:italic>\u2009=\u2009166) and a validation cohort (VC; <jats:italic>n<\/jats:italic>\u2009=\u2009250) of untreated chronic lymphocytic leukemia (CLL) patients. In the VC, novel mitogens were used to improve metaphase generation and <jats:italic>TP53<\/jats:italic>, <jats:italic>NOTCH1<\/jats:italic>, and <jats:italic>SF3B1<\/jats:italic> mutations were assessed. KA undetected by FISH were found in 35 and 35% of the cases in the LC and VC, respectively. In addition to FISH, KA allowed reclassification of 23 and 26% of cases in the LC and VC, respectively, into a higher cytogenetic risk group. By multivariate analysis, both in the LC and VC, KA other than isolated 13q deletion correlated with a shorter time to first treatment (TFT; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 and 0.003, respectively), while a complex karyotype predicted a worse overall survival (OS, <jats:italic>P<\/jats:italic>\u2009=\u20090.015 and 0.010, respectively). In the VC, where a comprehensive biologic assessment was performed, a shorter TFT was also predicted by stage (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), <jats:italic>IGHV<\/jats:italic> mutational status (<jats:italic>P<\/jats:italic>\u2009=\u20090.05), and del(17p)\/<jats:italic>TP53<\/jats:italic> mutations (<jats:italic>P<\/jats:italic>\u2009=\u20090.033) while stage (<jats:italic>P<\/jats:italic>\u2009=\u20090.023) and del(17p)\/<jats:italic>TP53<\/jats:italic> mutations (<jats:italic>P<\/jats:italic>\u2009=\u20090.024) independently predicted a shorter OS. FISH results did not independently impact on TFT and OS, in the LC and VC cohorts; this was also the case for <jats:italic>NOTCH1<\/jats:italic> and <jats:italic>SF3B1<\/jats:italic> mutations in the VC. We suggest that in CLL, conventional karyotyping with novel mitogens could be more effective than FISH for the detection of KA allowing for a more precise refinement of prognosis. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22293","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T17:39:34Z","timestamp":1441906774000},"page":"818-826","source":"Crossref","is-referenced-by-count":35,"title":["Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia: Clinical and biologic correlations"],"prefix":"10.1002","volume":"54","author":[{"given":"Gian Matteo","family":"Rigolin","sequence":"first","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"del Giudice","sequence":"additional","affiliation":[{"name":"Section of Hematology, Department of Cellular Biotechnologies and Hematology Policlinico Umberto I \u2018Sapienza\u2019 University Roma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Formigaro","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Saccenti","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Martinelli","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Cavallari","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Lista","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Tammiso","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleonora","family":"Volta","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. 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Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Sofritti","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Daghia","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. Anna, University of Ferrara  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Cavazzini","sequence":"additional","affiliation":[{"name":"Section of Hematology Azienda Ospedaliero\u2010Universitaria Arcispedale S. 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