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To reveal novel molecular factors behind the development of salivary gland cancer, we performed gene expression analyses from <jats:italic>Smgb<\/jats:italic>\u2010Tag mouse salivary gland samples. The overall purpose was to apply these results for clinical use to find new approaches for both possible therapeutic targets and more accurate diagnostic tools. <jats:italic>Smgb<\/jats:italic>\u2010Tag mouse strain, in which salivary neoplasms arise through a dysplastic phase in submandibular glands, was investigated using genome\u2010wide microarray expression analysis, ingenuity pathway analysis, RT\u2010PCR, and immunohistochemistry. Thirty\u2010eight human salivary gland adenoid cystic carcinoma samples were investigated using immunohistochemistry for validation purposes. Our genome\u2010wide study showed that <jats:italic>Ppp2r1b<\/jats:italic>, a PP2A subunit encoding tumor suppressor gene, is underexpressed in submandibular gland tumors of <jats:italic>Smgb\u2010<\/jats:italic>Tag mice. mTOR signaling pathway was significantly enriched and mTOR linked PP2A subunit gene B55 gamma was significantly underexpressed in the analyses. Furthermore, parallel immunohistochemical analysis of three PP2A inhibitors demonstrated that two PP2A inhibitors, CIP2A and SET, are highly expressed in both dysplastic and adenocarcinomatous tumors of the <jats:italic>Smgb\u2010<\/jats:italic>Tag mice. In addition, all 38 investigated human salivary adenoid cystic carcinoma samples stained positively for CIP2A and most for SET. Finally, p\u2010S6 staining showed activation of mTOR pathway in human adenoid cystic carcinoma samples. Our results suggest that PP2A inhibition either via PP2A subunit underexpression or PP2A inhibitor overexpression play an important role in the formation of salivary gland malignancy, potentially due to mTOR signaling activation. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/gcc.22312","type":"journal-article","created":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T12:51:17Z","timestamp":1443012677000},"page":"69-81","source":"Crossref","is-referenced-by-count":8,"title":["Potential role for inhibition of protein phosphatase 2A tumor suppressor in salivary gland malignancies"],"prefix":"10.1002","volume":"55","author":[{"given":"Johannes","family":"Routila","sequence":"first","affiliation":[{"name":"The Centre for Biotechnology University of Turku and \u00c5bo Akademi University Tykist\u00f6katu BioCity Turku FI\u201020521 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