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Chromosome instability (CIN) is an aberrant phenotype that occurs in \u223c80% of all cancer types and is associated with aggressive tumors, the acquisition of multidrug resistance and poor patient prognosis. Despite these associations however, the aberrant genes and molecular defects underlying CIN remain poorly understood. KIF11 is an evolutionarily conserved microtubule motor protein that functions in centrosome and chromosome dynamics in mitosis. Interestingly, the yeast ortholog of <jats:italic>KIF11<\/jats:italic>, namely <jats:italic>CIN8<\/jats:italic> is a CIN gene and thus aberrant KIF11 expression and function is suspected to underlie CIN. In support of this possibility, <jats:italic>KIF11<\/jats:italic> is somatically altered in a large number of cancer types. Using a complementary biochemical and genetic approach we examined whether <jats:italic>KIF11<\/jats:italic> silencing with siRNAs or inhibition with monastrol was able to convert two distinct and karyotypically stable cell lines into karyotypically unstable cell lines. Indeed, quantitative imaging microscopy and flow cytometry revealed that <jats:italic>KIF11<\/jats:italic> silencing induced increases in nuclear areas, micronucleus formation, DNA content and chromosome numbers relative to controls that was also observed following KIF11 inhibition. Collectively, this study identifies and validates <jats:italic>KIF11<\/jats:italic> as an evolutionarily conserved CIN gene, and further suggests that aberrant expression and function may contribute to the pathogenesis of a subset of cancers.<\/jats:p>","DOI":"10.1002\/gcc.22471","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T16:26:39Z","timestamp":1494951999000},"page":"668-680","source":"Crossref","is-referenced-by-count":37,"title":["<i>KIF11<\/i> silencing and inhibition induces chromosome instability that may contribute to cancer"],"prefix":"10.1002","volume":"56","author":[{"given":"Yasamin","family":"Asbaghi","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Medical Genetics University of Manitoba Winnipeg Manitoba Canada"},{"name":"Research Institute in Oncology and Hematology, CancerCare Manitoba Winnipeg Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura 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