{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:35:32Z","timestamp":1781822132751,"version":"3.54.5"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:00:00Z","timestamp":1698451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High\u2010grade B\u2010cell lymphoma (HGBL)\/diffuse large B\u2010cell lymphoma (DLBCL) with rearrangements (R) in <jats:italic>MYC<\/jats:italic> and <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> are correlated with poor prognosis. Little is known about the impact of other genetic alterations (gain (G) or amplification (A)) of these genes. The aim of the study was to investigate whether we can identify new prognostic subgroups. Fluorescence in situ hybridization (FISH) results from 169 HGBL\/DLBCL were retrospectively categorized into: (1) concurrent <jats:italic>MYC<\/jats:italic>\u2010R and <jats:italic>BCL2<\/jats:italic>\u2010R and\/or <jats:italic>BCL6<\/jats:italic>\u2010R\u2014samples with <jats:italic>MYC<\/jats:italic>\u2010R and <jats:italic>BCL2<\/jats:italic>\u2010R (+\/\u2212 <jats:italic>BCL6<\/jats:italic>\u2010R); <jats:italic>n<\/jats:italic>\u2009=\u200921, and HGBL\/DLBCL with <jats:italic>MYC<\/jats:italic>\u2010R and <jats:italic>BCL6<\/jats:italic>\u2010R; <jats:italic>n<\/jats:italic>\u2009=\u200911; (2) concurrent R and G\/A in <jats:italic>MYC<\/jats:italic> and <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> called \u201calternative HGBL\/DLBCL\u201d\u2014samples with (<jats:italic>n<\/jats:italic>\u2009=\u200916) or without (<jats:italic>n<\/jats:italic>\u2009=\u20096) <jats:italic>BCL2<\/jats:italic> involvement; (3) <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> alterations without <jats:italic>MYC<\/jats:italic> involvement (<jats:italic>n<\/jats:italic>\u2009=\u200935); (4) concurrent G\/A in <jats:italic>MYC<\/jats:italic> and <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> without R (<jats:italic>n<\/jats:italic>\u2009=\u200925); and (5) \u201cNo alterations\u201d (<jats:italic>n<\/jats:italic>\u2009=\u200955). Patients with HGBL\/DLBCL\u2010<jats:italic>MYC\/BCL2<\/jats:italic> and \u201calternative\u201d HGBL\/DLBCL (with <jats:italic>BCL2<\/jats:italic> involvement) had significantly worse survival rates compared to the \u201cno alterations\u201d group. G\/A of these genes in the absence of rearrangements did not show any prognostic significance. HGBL\/DLBCL with <jats:italic>MYC<\/jats:italic>\u2010R and <jats:italic>BCL6<\/jats:italic>\u2010R without <jats:italic>BCL2<\/jats:italic> involvement showed a better survival rate compared to HGBL\/DLBCL\u2010<jats:italic>MYC\/BCL2<\/jats:italic>. According to immunohistochemistry, \u201cdouble\/triple\u201d expression (DEL\/TEL) did not show a significantly worse outcome compared to absent DEL\/TEL. This study highlights the continued value of FISH assessment of <jats:italic>MYC<\/jats:italic>, <jats:italic>BCL2,<\/jats:italic> and <jats:italic>BCL6<\/jats:italic> in the initial evaluation of HGBL\/DLBCL with different survival rates between several genetic subgroups.<\/jats:p>","DOI":"10.1002\/gcc.23211","type":"journal-article","created":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T11:15:19Z","timestamp":1698491719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Alternative genetic alterations of <i>MYC<\/i>, <i>BCL2<\/i>, and\/or <i>BCL6<\/i> in high\u2010grade B\u2010cell lymphoma (<scp>HGBL<\/scp>) and diffuse large B\u2010cell lymphoma (<scp>DLBCL<\/scp>): Can we identify different prognostic subgroups?"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6128-2027","authenticated-orcid":false,"given":"Siska","family":"Blomme","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"De Paepe","sequence":"additional","affiliation":[{"name":"Department of Pathology AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Devos","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Emmerechts","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Snauwaert","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Cauwelier","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine AZ Sint\u2010Jan Hospitals Brugge\u2010Oostende  Brugge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,28]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2022"},{"key":"e_1_2_10_3_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow 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