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This implies an important role for chromosome 21 in the pathobiology of BCPALL, emphasized by the increased risk of BCPALL in children with Down syndrome. However, model systems of chromosome 21 gain are lacking. We therefore developed a BCPALL cell line (Nalm\u20106, <jats:italic>DUX4<\/jats:italic>\u2010rearranged) with an additional chromosome 21 by means of microcell\u2010mediated chromosome transfer. FISH, PCR, multiplex ligation\u2010dependent probe amplification, and whole exome sequencing showed that an additional chromosome 21 was successfully transferred to the recipient cells. Transcription of some but not all genes on chromosome 21 was increased, indicating tight transcriptional regulation. Nalm\u20106 cells with an additional chromosome 21 proliferated slightly slower compared with parental Nalm\u20106 and sensitivity to induction chemotherapeutics was mildly increased. The extra copy of chromosome 21 did not confer sensitivity to targeted signaling inhibitors. In conclusion, a BCPALL cell line with an additional human chromosome 21 was developed, validated, and subjected to functional studies, which showed a minor but potentially relevant effect in vitro. This cell line offers the possibility to study further the role of chromosome 21 in ALL.<\/jats:p>","DOI":"10.1002\/gcc.23217","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T07:24:45Z","timestamp":1702452285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The impact of an additional copy of chromosome 21 in <scp>B\u2010cell<\/scp> precursor acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"63","author":[{"given":"Femke M.","family":"Hormann","sequence":"first","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht The Netherlands"},{"name":"Department of Pediatric Oncology\/Hematology Erasmus Medical Center \u2010 Sophia Children's Hospital  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva J.","family":"Mooij","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marieke","family":"van de Mheen","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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