{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:42:31Z","timestamp":1786977751254,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006058","name":"St. Baldrick's Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006058","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017050","name":"Cycle for Survival","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50CA217694"],"award-info":[{"award-number":["P50CA217694"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30CA008748"],"award-info":[{"award-number":["P30CA008748"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Kinase fusion\u2010positive soft tissue tumors represent an emerging, molecularly defined group of mesenchymal tumors with a wide morphologic spectrum and diverse activating kinases. Here, we present two cases of soft tissue tumors with novel\n                      <jats:italic>LTK<\/jats:italic>\n                      fusions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      Both cases presented as acral skin nodules (big toe and middle finger) in pediatric patients (17\u2010year\u2010old girl and 2\u2010year\u2010old boy). The tumors measured 2 and 3\u2009cm in greatest dimension. Histologically, both cases exhibited bland\u2010looking spindle cells infiltrating adipose tissue and accompanied by collagenous stroma. One case additionally displayed perivascular hyalinization and band\u2010like stromal collagen. Both cases exhibited focal S100 staining, and one case had patchy coexpression of CD34. Targeted RNA\u2010seq revealed the presence of novel in\u2010frame\n                      <jats:italic>MYH9::LTK<\/jats:italic>\n                      and\n                      <jats:italic>MYH10::LTK<\/jats:italic>\n                      fusions, resulting in upregulation of\n                      <jats:italic>LTK<\/jats:italic>\n                      expression. Of interest, DNA methylation\u2010based unsupervised clustering analysis in one case showed that the tumor clustered with dermatofibrosarcoma protuberans (DFSP). One tumor was excised with amputation with no local recurrence or distant metastasis at 18\u2010month follow\u2010up. The other case was initially marginally excised with local recurrence after one year, followed by wide local excision, with no evidence of disease at 10\u2009years of follow\u2010up.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This is the first reported case series of soft tissue tumors harboring\n                      <jats:italic>LTK<\/jats:italic>\n                      fusion, expanding the molecular landscape of soft tissue tumors driven by activating kinase fusions. Furthermore, studies involving a larger number of cases and integrated genomic analyses will be warranted to fully elucidate the pathogenesis and classification of these tumors.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/gcc.23227","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T08:16:25Z","timestamp":1711095385000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Spindle cell neoplasms with novel\n                    <i>LTK<\/i>\n                    fusion \u2013 Expanding the spectrum of kinase fusion\u2010positive soft tissue tumors"],"prefix":"10.1002","volume":"63","author":[{"given":"Maximus C. F.","family":"Yeung","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine The University of Hong Kong  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine K.","family":"Dermawan","sequence":"additional","affiliation":[{"name":"Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony P. Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Adolescent Medicine, School of Clinical Medicine The University of Hong Kong  Hong\u00a0Kong"},{"name":"Department of Paediatrics and Adolescent Medicine Hong Kong Children's Hospital  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert Y. L.","family":"Lam","sequence":"additional","affiliation":[{"name":"Division of General Orthopaedics and Oncology, Department of Orthopedics and Traumatology, School of Clinical Medicine The University of Hong Kong  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9717-8205","authenticated-orcid":false,"given":"Cristina R.","family":"Antonescu","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony W. H.","family":"Shek","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine The University of Hong Kong  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22846"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22877"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22671"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2350\/10-12-0944-PB.1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4701"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-00657-x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04135-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1842-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.257246.119"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3729"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0175-7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu049"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20603-4"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/10668969221080059"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.014"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201153"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031733"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201903068"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00455-9"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208506200"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10232"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08994"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1086\/324267"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2016.09.013"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.14148"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-021-01123-1"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0089-4"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22663"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/dermatopathology10010008"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30119-0"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.23227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T09:59:09Z","timestamp":1711533549000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.23227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/gcc.23227"],"URL":"https:\/\/doi.org\/10.1002\/gcc.23227","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2023-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23227"}}