{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T14:24:39Z","timestamp":1700317479406},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":6160,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report the sublocalization of the breakpoint in chromosome 22 in 33 patients with chronic myeloid leukemia (CML) who also had unusual marrow cytogenetics. In 23 patients, the leukemic clones were characterized by Philadelphia (Ph<jats:sup>1<\/jats:sup>) chromosomes that arose through complex translocations that involved three or more chromosomes. In the remaining ten patients, there were no detectable Ph<jats:sup>1<\/jats:sup> chromosomes despite molecular evidence for the presence of rearrangements in the major breakpoint cluster region (<jats:italic>bcr<\/jats:italic>) of chromosome 22 in all cases. There was no significant difference between the two groups with respect to location of the breakpoints within the <jats:italic>bcr.<\/jats:italic> When these two groups of patients were combined, there was a significant excess of breakpoints in one segment of the <jats:italic>bcr<\/jats:italic> when compared to the distribution of breakpoints seen in 119 patients with simple 9;22 translocations. The difference in breakpoint distributions did not appear to be entirely attributable to differences between groups in disease duration at the time of study. These data support the notion that the unusual genetic recombinations that give rise to <jats:italic>BCR\/ABL<\/jats:italic> fusion genes in CML involve specific DNA sequences of <jats:italic>BCR<\/jats:italic> (and possibly <jats:italic>ABL<\/jats:italic>) and additional, recombinogenic sequences, at least some of which are present in loci known to be nonrandomly involved in complex Ph<jats:sup>1<\/jats:sup> translocations.<\/jats:p>","DOI":"10.1002\/gcc.2870010116","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T22:38:35Z","timestamp":1172097515000},"page":"106-111","source":"Crossref","is-referenced-by-count":30,"title":["Location of breakpoints within the major breakpoint cluster region (<i>bcr<\/i>) in 33 patients with <i>bcr<\/i> rearrangement\u2010positive chronic myeloid leukemia (CML) with complex or absent Philadelphia chromosomes"],"prefix":"10.1002","volume":"1","author":[{"given":"Ian","family":"Dub\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Dixon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Beckett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Grossman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Weinstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Benn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"McKeithan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Con","family":"Norman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Pinkerton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(87)90025-2"},{"key":"e_1_2_1_3_1","first-page":"20","article-title":"Cytogenetics of chronic myelogenous leukemia","volume":"25","author":"Bernstein R","year":"1988","journal-title":"Semin Hematol"},{"key":"e_1_2_1_4_1","first-page":"648","article-title":"Clinical evaluation of a DNA probe assay for the Philadelphia (Ph1) translocation in chronic myelogenous leukemia","volume":"2","author":"Blennerhasset GT","year":"1988","journal-title":"Leukemia"},{"key":"e_1_2_1_5_1","first-page":"642","article-title":"The location of breakpoints within the breakpoint cluster region bcr of chromosome 22 in chronic myeloid leukemia","volume":"2","author":"Eisenberg A","year":"1988","journal-title":"Leukemia"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90083-9"},{"key":"e_1_2_1_7_1","unstructured":"ISCN[Harnden DG Klinger HP (eds)]An International System for Human Cytogenetic Nomenclature. Basel: Karger. Published in collaboration with Cytogenet Cell Genet; also in Birth Defects: Original Articles Series Vol 21 No. 1. New York: March of Dimes Birth Defects Foundation 1985."},{"key":"e_1_2_1_8_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1182\/blood.V72.4.1237.1237","article-title":"The site of the breakpoint within the bcr is a prognostic factor in Philadelphia\u2010positive CML patients","volume":"72","author":"Mills KI","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_10_1","volume-title":"Catalog of Chromosome Aberrations in Cancer","author":"Mitelman F","year":"1988"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90240-3"},{"key":"e_1_2_1_12_1","volume-title":"Human Cytogenetics: A Practical Approach","author":"Rooney DE","year":"1986"},{"key":"e_1_2_1_13_1","first-page":"82","volume-title":"Genetic Rearrangements in Leukemia and Lymphoma. Leukemia and Lymphoma Research 2","author":"Rowley JD","year":"1986"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1182\/blood.V70.2.448.448","article-title":"CML patients in blast crisis have breakpoints localized to a specific region of the BCR","volume":"70","author":"Schaefer\u2010Rego K","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1182\/blood.V72.2.485.485","article-title":"Analysis of breakpoints within the bcr gene and their correlation with the clinical course of Philadelphia\u2010positive chronic myelogenous leukemia","volume":"72","author":"Shtalrid M","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(87)90068-3"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90244-0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1182\/blood.V71.2.349.349","article-title":"The correlation of breakpoint cluster rearrangement and p210 ph1\/abl expression with morphological analysis of Ph\u2010negative chronic myeloid leukemia and their myeloproliferative diseases","volume":"71","author":"Wiedemann LM","year":"1988","journal-title":"Blood"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870010116","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870010116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T05:57:46Z","timestamp":1697954266000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870010116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,9]]}},"alternative-id":["10.1002\/gcc.2870010116"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870010116","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,9]]}}}