{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T14:01:51Z","timestamp":1698242511347},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":5430,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chromosomal in situ suppression (CISS) hybridization was used to investigate the distribution of material of chromosomes 1 and 5 present in marker chromosomes of Namalva cells. The Namalva cell line, established from a Burkitt's lymphoma, exhibits a highly variable female karyotype with a large number of marker chromosomes. Libraries from sorted human chromosomes 1 and 5 were used to delineate material of these chromosomes present in the Namalva karyotype. We used the DAB\/peroxidase reaction and reflection contrast microscopy for detection of biotinylated hybrid molecules. Identification of chromosomes was achieved by fluorescent R\u2010banding after CISS hybridization, which allowed the assignment of hybridized regions to the particular marker chromosomes. After CISS hybridization with a chromosome I library, the normal chromosome I was labelled as well as a large marker MI and the long arm of marker M3. Using a chromosome 5 library it could be shown that the distal part of the long arm of one chromosome 5 was translocated to marker M2. The normal chromosome 5 was completely labelled. The present investigation demonstrates the advantage of combining CISS hybridization and banding for the identification of complex, rearranged tumor karyotypes.<\/jats:p>","DOI":"10.1002\/gcc.2870030511","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T22:50:12Z","timestamp":1172098212000},"page":"394-399","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of Marker Chromosomes in Namalva Cells by Chromosomal In Situ Suppression (CISS) Hybridization and R\u2010Banding"],"prefix":"10.1002","volume":"3","author":[{"given":"Peter","family":"Ruppersberger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Zankl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Scherthan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"10","article-title":"Non\u2010radioactive in situ hybridization and chromosome banding","volume":"7","author":"Ambros P","year":"1987","journal-title":"Ann Univ Sarav Med Suppl"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01790091"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990110113"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.12.4761"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00193186"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990110112"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/33.12.2415575"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00291428"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01790090"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(89)90011-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.9138"},{"key":"e_1_2_1_13_1","unstructured":"RaederU(1986)Molecular genetics of lignin degrading fungi. Ph.D. thesis University of Manchester GB."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5650"},{"key":"e_1_2_1_15_1","unstructured":"ScherthanH BauerS ZanklH(1989)Molecular studies by non\u2010isotopic in situ hybridization in Namalva cells. The 10th International Chromosome Conference Uppsala Sweden."},{"key":"e_1_2_1_16_1","first-page":"61","article-title":"Application of reflection contrast and fluorescence microscopy to non\u2010radioactive in situ hybridization","volume":"39","author":"Scherthan H","year":"1990","journal-title":"Lab Prac"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132947"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(87)90103-0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90292-3"},{"key":"e_1_2_1_20_1","first-page":"393","article-title":"Long term cultivation of Namalva cells for interferon production: stable cytogenetic markers for identification of cells in spite of drastic chromosomal variation","volume":"60","author":"Wurm F","year":"1985","journal-title":"Dev Biol Stand"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870030511","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870030511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T22:14:39Z","timestamp":1698012879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870030511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,9]]}},"alternative-id":["10.1002\/gcc.2870030511"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870030511","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9]]}}}