{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:49:31Z","timestamp":1757778571421},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4730,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1993,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parathyroid hormone\u2010related protein (PTHRP) is expressed in a large number of tumors and is the mediator of parathyroid hormone\u2010like effects seen in humoral hypercalcemia of malignancy. The gene coding for <jats:italic>PTHRP<\/jats:italic> has been localised to the short arm of chromosome 12. This is at the same region as the oncogene <jats:italic>KRAS2<\/jats:italic>, and amplification of <jats:italic>KRAS2<\/jats:italic> has previously been found in human lung cancer. The BEN cell line which is known to express PTHRP was established from a patient who had squamous cell carcinoma of the lung with hypercalcemia. Cytogenetic analysis of the BEN cell line revealed a very complex karyotype with many marker chromosomes. Chromosomal in situ hybridization with biotinylated DNA probes visualized by a biotin\u2010streptavidin\u2010polyalkaline\u2010phosphatase complex was used to analyse two dicentric marker chromosomes containing homogeneously staining regions (hsr) in BEN. The hsr were found to contain amplified <jats:italic>PTHRP<\/jats:italic> and <jats:italic>KRAS2<\/jats:italic> at levels of 30\u2010fold and 14\u2010fold per cell, respectively. The higher level of amplification of the <jats:italic>PTHRP<\/jats:italic> gene would suggest that <jats:italic>PTHRP<\/jats:italic> is the target gene of amplification in the amplicon. This is the first report of gene amplification of <jats:italic>PTHRP<\/jats:italic> and in addition its co\u2010amplification with <jats:italic>KRAS2<\/jats:italic>. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.2870070406","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T23:38:56Z","timestamp":1172101136000},"page":"213-218","source":"Crossref","is-referenced-by-count":10,"title":["Co\u2010amplification of the gene for parathyroid hormone\u2010related protein (PTHRP) and <i>KRAS2<\/i> in a human lung cancer cell line"],"prefix":"10.1002","volume":"7","author":[{"given":"Christina","family":"Rudduck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob","family":"Center","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. Margaret","family":"Garson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"413","article-title":"Chromosomal localization of the hst oncogene and its co\u2010amplification with the int.2 oncogene in a human melanoma","volume":"2","author":"Adelaide J","year":"1988","journal-title":"Oncogene"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(77)90089-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1975.237"},{"key":"e_1_2_1_5_1","first-page":"1693","article-title":"Association of Ki\u2010ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in\u2010gel renaturation assay","volume":"49","author":"Fukumoto M","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132006"},{"key":"e_1_2_1_7_1","first-page":"2934","article-title":"Localization of the human insulin gene to the distal end of the short arm of chromosome 11","volume":"83","author":"Harper ME","year":"1981","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.2.163"},{"key":"e_1_2_1_9_1","first-page":"467","article-title":"Parathyroid hormone\u2010related protein: Isolation, molecular cloning and mechanism of action","volume":"45","author":"Martin TJ","year":"1989","journal-title":"Recent Prog Horm Res"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00333988"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2934"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(92)90134-T"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870020105"},{"key":"e_1_2_1_14_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870010302"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.11.3761"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3616618"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90363-6"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02236.x"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(83)90044-4"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.13.4861"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/312521a0"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870070406","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870070406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:40:52Z","timestamp":1698226852000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870070406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,8]]}},"alternative-id":["10.1002\/gcc.2870070406"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870070406","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,8]]}}}