{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:17:16Z","timestamp":1773339436311,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4608,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leukemic cells from two patients with Philadelphia\u2010negative chronic myeloid leukemia (CML) were investigated: I) Cytogenetics showed a normal 46.XY karyotype in both cases, 2) molecular studies revealed rearrangement of the M\u2010BCR region and formation of <jats:italic>BCR\u2010ABL<\/jats:italic> fusion mRNA with b2a2 (patient I) or b3a2 (patient 2) configuration, and 3) fluorescence in situ hybridization (FISH) demonstrated relocation of the 5\u2032 <jats:italic>BCR<\/jats:italic> sequences from one chromosome 22 to one chromosome 9. The <jats:italic>ABL<\/jats:italic> probe hybridized to both chromosomes 9 at band q34, while two other probes which map centromeric and telomeric of <jats:italic>BCR<\/jats:italic> on 22q 11 hybridized solely with chromosome 22. For the first time, a <jats:italic>BCR\u2010ABL<\/jats:italic> rearrangement is shown to take place on 9q34 instead of in the usual location on 22q 11. A rearrangement in the latter site is found in all Ph\u2010positive CML and in almost all investigated CML with variant Ph or Ph\u2010negative, <jats:italic>BCR<\/jats:italic>\u2010positive cases. The few aberrant chromosomal localizations of <jats:italic>BCR\u2010ABL<\/jats:italic> recombinant genes found previously were apparently the result of complex and successive changes. Furthermore in patient 2, both chromosomes 9 showed positive FISH signals with both <jats:italic>ABL<\/jats:italic> and <jats:italic>BCR<\/jats:italic> probes. Restriction fragment length polymorphism (RFLP) analysis indicated that mitotic recombination had occurred on the long arm of chromosome 9 and that the rearranged chromosome 9 was of paternal origin. The leukemic cells of this patient showed a duplication of the <jats:italic>BCR\u2010ABL<\/jats:italic> gene, analogous to duplication of the Ph chromosome in classic CML. In addition they had lost the maternal alleles of the 9q34 chromosomal region. The lymphocytes of patient 2 carried the maternal chromosome 9 alleles and were Ph\u2010negative as evidenced by RFLP and FISH analyses, respectively. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.2870080406","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T23:51:58Z","timestamp":1172101918000},"page":"237-245","source":"Crossref","is-referenced-by-count":79,"title":["Translocation of <i>BCR<\/i> to chromosome 9: A new cytogenetic variant detected by FISH in two Ph\u2010negative, <i>BCR<\/i>\u2010positive patients with chronic myeloid leukemia"],"prefix":"10.1002","volume":"8","author":[{"given":"Anne","family":"Hagemeijer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjan","family":"Buijs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Smit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert\u2010Jan","family":"Creemers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorien Van Der","family":"Plas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard","family":"Grosveld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132972"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/j.1460-2075.1985.tb03683.x","article-title":"c\u2010abl and bcr are rearranged in a Ph1\u2010negative CML patient","volume":"4","author":"Bartram CR","year":"1985","journal-title":"EMBO J"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)90594-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870010116"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1182\/blood.V68.4.957.957","article-title":"Rearrangement of the brc gene in Philadelphia chromosome negative chronic myeloid leukemia","volume":"68","author":"Ganesan TS","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/359414a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000131327"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90083-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(85)90059-7"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/315758a0"},{"key":"e_1_2_1_13_1","first-page":"628","article-title":"Bcr\u2010abl oncogene activation in Philadelphia chromosome positive acute lymphoblastic leukemia","volume":"2","author":"Hermans A","year":"1988","journal-title":"Leukemia"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286701"},{"key":"e_1_2_1_15_1","volume-title":"Guidelines for Cancer Cytogenetics. Supplement to an International System for Human Cytogenetic Nomenclature","author":"ISCN","year":"1991"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-105-5-673"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1182\/blood.V78.1.205.205","article-title":"Philadelphia\u2010negative (Ph\u2013) chronic myeloid leukemia (CML): Comparison with Ph(+) CML and chronic myelomonocytic leukemia","volume":"78","author":"Martiat P","year":"1991","journal-title":"Blood"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/320281a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90240-3"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1182\/blood.V76.9.1812.1812","article-title":"Ph\u2010negative chronic myeloid leukemia: Molecular analysis of ABL,insertion into M\u2010BCR on chromosome 22","volume":"76","author":"Morris CM","year":"1990","journal-title":"Blood"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870030405"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1992.tb01145.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1182\/blood.V72.1.24.24","article-title":"Acquired homozygosity of the rearranged bcr allele during the acute leukemic phase of a patient with Ph\u2010negative chronic myeloid leukemia","volume":"72","author":"Reeve AE","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1182\/blood.V73.4.1038.1038","article-title":"Cytogenetic and molecular analysis in Philadelphia negative CML","volume":"73","author":"Van der Plas DC","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(91)90457-6"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870080406","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870080406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T23:42:34Z","timestamp":1698277354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870080406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1002\/gcc.2870080406"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870080406","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}