{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:55:47Z","timestamp":1761918947511},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4518,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sixty consecutive evaluable specimens from patients with non\u2010Hodgkin's lymphoma (NHL) were studied for the incidence of polysomy of chromosome 12 by fluorescence in situ hybridization (FISH) with probes for the repetitive DNA sequence in the centromeric region of chromosome 12. Thirty\u2010six samples were from follicular lymphomas (FLs), and twenty\u2010four were from diffuse large cell lymphomas (DLCLs). Fifty\u2010two specimens (86%) were obtained by fine\u2010needle aspiration of a diseased node, seven (11.6%) were from involved bone marrows, and one specimen was from a pleural effusion. Twelve of the thirty\u2010six (33%) cases with FL had trisomy 12 in 3\u201041% of the cells (median, 10%) (normal controls had three signals in 1.4 +M 0.7% of cells). Trisomy 12 was found in 62% of the patients who had had FL for more than 5 years. Nine of the twenty\u2010four cases (37%) with DLCL had more than two copies of chromosome 12 in 4\u201092% of the cells (median, 78%), and all nine cases were of B\u2010cell phenotype. Unlike FL cells, some DLCL cells had 4\u20106 copies of chromosome 12. In previously untreated patients, 54% of DLCLs and 26% of FLs had subpopulations of cells containing more than two copies of chromosome 12 (<jats:italic>P<\/jats:italic> = 0.04). Only 2\/7 cases of DLCL with polysomy 12 had rearrangement of the <jats:italic>BCL<\/jats:italic>2 gene, indicating that the majority of DLCL cases with polysomy 12 did not result from histologic transformation of low grade follicular lymphomas. These data demonstrate that FISH of interphase cells is a sensitive method for detecting numerical abnormalities of chromosome 12 in NHL. The differences between FL and DLCL in the number of chromosomes 12 and the number of involved cells may be related to differences in the biology of these tumors. <jats:italic>Genes Chrom Cancer<\/jats:italic> 9:161\u2010167 (1994). \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.2870090303","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T00:16:42Z","timestamp":1172103402000},"page":"161-167","source":"Crossref","is-referenced-by-count":16,"title":["Polysomy of chromosome 12 in 60 patients with non\u2010hodgkin's lymphoma assessed by fluorescence in situ hybridization: Differences between follicular and diffuse large cell lymphoma"],"prefix":"10.1002","volume":"9","author":[{"given":"Anas","family":"Younes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Goodacre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Andreeff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Pugh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Katz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debbie","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Cabanillas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1182\/blood.V79.7.1796.1796","article-title":"Detection of trisomy 12 in chronic lymphocytic leukemia by fluorescence in situ hybridization to interphase cells: A simple and sensitive method","volume":"79","author":"Anastasi J","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_3_1","first-page":"725","volume-title":"Flow Cytometry and Cell Sorting","author":"Andreeff M","year":"1990"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1986.tb42053.x"},{"key":"e_1_2_1_5_1","first-page":"5557","article-title":"Frequent nonrandom chromosome abnormalities in 27 patients with untreated large cell lymphoma and immunoblastic lymphoma","volume":"48","author":"Cabanillas F","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb08206.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1182\/blood.V81.10.2702.2702","article-title":"Fluorescent in situ hybridization (FISH) and cytogenetic studies of trisomy 12 in chronic lymphocytic leukemia","volume":"81","author":"Escudier SM","year":"1993","journal-title":"Blood"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(92)90366-G"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(92)90230-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb07877.x"},{"key":"e_1_2_1_11_1","first-page":"196a","article-title":"Interphase fluorescent in situ hybridization for trisomy 12 substantiates the utility of mitogens in the cytogenetic analysis of low grade lymphoproliferative processes","volume":"80","author":"Kubic VL","year":"1992","journal-title":"Blood"},{"key":"e_1_2_1_12_1","first-page":"20","article-title":"Chromosomal abnormalities in non\u2010Hodgkin's lymphoma","volume":"17","author":"Le Beau M","year":"1990","journal-title":"Semin Oncol"},{"key":"e_1_2_1_13_1","first-page":"7","article-title":"Cytogenetics of non\u2010Hodgkin's lymphoma","volume":"10","author":"Levine EG","year":"1990","journal-title":"Monogr Natl Cancer Inst"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90146-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870030304"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1182\/blood.V78.3.775.775","article-title":"Trisomy 12 in chronic lymphocytic leukemia: An interphase cytogenetic study","volume":"78","author":"Perez\u2010Losada A","year":"1991","journal-title":"Blood"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2934"},{"key":"e_1_2_1_18_1","first-page":"1959","article-title":"Interphase cytogenetics of hematologic cancer: Comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes","volume":"51","author":"Poddighe PJ","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_19_1","first-page":"602","article-title":"Interphase detection of trisomy 12 in B\u2010cell chronic lymphocytic leukemia by fluorescence hybridization in situ","volume":"6","author":"Qumsiyeh MB","year":"1992","journal-title":"Leukemia"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(92)90005-S"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/oxfordjournals.annonc.a058361","article-title":"The clinical relevance of t(14;18)\/BCL\u20102rearrangement and del 6q in diffuse large cell lymphoma and immunoblastic lymphoma","volume":"4","author":"Romaguera JE","year":"1993","journal-title":"Ann Oncol"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1182\/blood.V75.9.1841.1841","article-title":"Chromosomal abnormalities in untreated patients with non\u2010Hodgkin's lymphoma: Association with histology, clinical characteristics, and treatment outcome","volume":"75","author":"Schouten HC","year":"1990","journal-title":"Blood"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60567-1"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870040405"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870090303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870090303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T04:57:36Z","timestamp":1698123456000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870090303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,3]]}},"alternative-id":["10.1002\/gcc.2870090303"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870090303","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3]]}}}