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Metaphases suitable for karyotyping were obtained in 70% of the cases; direct harvest yielded metaphases in 29% and cultures without and with digestion in 40% and 59%, respectively. Chromosomal abnormalities were detected in 37 cases. Cells judged to be phenotypically abnormal in culture were twice as likely to reveal chromosomal aberrations as normal\u2010looking cells. Eight cases showed multiclonal abnormalities. Significant differences were detected in the karyotypic profile depending on the method used. With direct harvest, the yield of complex chromosomal changes was 87%, compared to 44% after culture of digested tissue (<jats:italic>P<\/jats:italic> &lt; 0.01), and also polyploidy was more common in direct\u2010harvested samples. Detailed karyotypic analysis was possible in 29 primary tumors. The chromosomes most frequently involved were 1, 3, 7, 11, 16, and 17. Recurrent structural abnormalities were der( 1; 16) (q 10; p 10), i(1)(q10), del(6)(q21), and del(1)(p22). Breakpoints clustered to the cententromere regions of chromosomes 1, 3, 11, 15, and 16 and to the short arms of chromosomes 7, 17, and 19. Seven of twenty\u2010nine fully analyzed cases had a family history of breast cancer, and changes of chromosomes 1, 3, and 15 seemed to be more common in these cases. There was an association between karyotype and survival: The 3 year survival was 63% in patients with complex karyotypic changes and 92% in those without complex changes. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.2870130403","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T00:10:14Z","timestamp":1172103014000},"page":"239-248","source":"Crossref","is-referenced-by-count":45,"title":["Cytogenetic studies of breast carcinomas: Different karyotypic profiles detected by direct harvesting and short\u2010term 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